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Regulation of Murine Osteoblast Macrophage Colony-Stimulating Factor Production by 1,25(OH)2D3
| Content Provider | Semantic Scholar |
|---|---|
| Author | Rubin, Janet Fan, Xuetao Thornton, David N. Bryant, R. Biskobing, Diane M. |
| Copyright Year | 1996 |
| Abstract | Abstract. Macrophage colony stimulating factor (MCSF) is important for formation of osteoclasts. We investigated the ability of 1,25(OH)2D3 to regulate osteoblast production of MCSF. Mouse calvarial osteoblasts were cultured for 2 days ± 1,25(OH)2D3. Since 1,25(OH)2D3 decreased osteoblast proliferation by 17.6 ± 1% at 10 nM and 11 ± 4% at 1 nM, the effect of growth rate on MCSF secretion was examined. Limiting cell proliferation by serum did not affect MCSF production. 1,25(OH)2D3 (1 nM) increased MCSF production (U/105 cells) maximally by 68 ± 33% (n = 3) with an ED50 for 1,25(OH)2D3 of 5 × 10−11 M. To investigate effects of 1,25(OH)2D3 on MCSF gene regulation, RT-PCR primers were designed to identify the mRNA coding for the membrane-bound isoform of MCSF. Simultaneous RT-PCR of glyceraldehyde-phosphate dehydrogenase (GAP) allowed semiquantitative assessment of MCSF mRNA between treatment groups expressed as the MCSF/GAP RT-PCR product ratio; both MCSF and GAP (+) primers were labeled with 32P-ATP for phosphorimage quantitation. The membrane-bound MCSF/GAP PCR product ratio was not affected by proliferative rate when growth was limited by [serum]. The MCSF/GAP RT-PCR product ratio was dose dependently increased by 1,25(OH)2D3, maximally at 1 nM at 2.2 ± 0.2 = fold (n = 10). 1,25 (OH)2D3 also increased the expression of an RT-PCR MCSF/GAP product ratio which represented the secreted isoform of MCSF. The ability of 1,25(OH)2D3 to pretranslationally regulate expression of membrane-bound osteoblast MCSF may be important in osteoblast:osteoclast interactions. |
| Starting Page | 291 |
| Ending Page | 296 |
| Page Count | 6 |
| File Format | PDF HTM / HTML |
| DOI | 10.1007/s002239900125 |
| PubMed reference number | 8781054 |
| Journal | Medline |
| Volume Number | 59 |
| Alternate Webpage(s) | https://page-one.springer.com/pdf/preview/10.1007/s002239900125 |
| Alternate Webpage(s) | https://doi.org/10.1007/s002239900125 |
| Journal | Calcified Tissue International |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
