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Comparison of conventional PCR with real-time PCR and branched DNA-based assays for hepatitis C virus RNA quantification and clinical significance for genotypes 1 to 5.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Sarrazin, Christoph Gärtner, Barbara Christine Sizmann, Dorothea Babiel, Rainer Mihm, Ulrike Hofmann, Wolf Peter Wagner, Michael Von Zeuzem, Stefan |
| Copyright Year | 2006 |
| Abstract | The key parameter for diagnosis and management of hepatitis C virus (HCV) infection is HCV RNA. Standardization of HCV RNA assays to IU is mainly based on genotype 1 panels. Little is known about the variability of commercially available HCV RNA assays for quantification of different genotypes. Two real-time reverse transcription (RT)-PCR assays (COBAS TaqMan HCV Test for use with the High-Pure System [HPS/CTM] and COBAS Ampliprep/COBAS TaqMan HCV Test [CAP/CTM]), one standard RT-PCR assay (COBAS Amplicor HCV Monitor 2.0 [CAM]), and one signal amplification assay (Versant Quantitative 3.0 [branched DNA [bDNA]]) were compared for quantification of genotypes 1 to 5 (n = 108). Using CAM as a reference assay for genotype 1-infected patients, the mean interassay differences compared with CAP/CTM, HPS/CTM, and bDNA were 0.16, -0.13, and -0.48 log(10) IU/ml HCV RNA, respectively. Comparison of CAM with CAP/CTM, HPS/CTM, and bDNA for the remaining genotypes showed the following results, respectively: 2a/c, -0.24, -0.78, and -0.49; 2b, -0.21, -0.18, and -0.64; 3a, 0.13, -1.04, and -0.55; 4, -0.52, -1.51, and -0.05; and 5, -0.28, -1.00, and -0.24 log IU/ml HCV RNA. A correct decision for treatment discontinuation in genotype 1 patients at week 12 was possible only when the same assay was used at baseline and week 12. Comparison of CAM with the CAP/CTM assay showed equal quantifications of genotype 1, 2, 3, and 5 samples, while genotype 4 samples were slightly underestimated. For the HPS/CTM assay, a significant underestimation of the HCV RNA concentrations of genotypes 2a/c, 3, 4, and 5 was observed. For the bDNA assay, a constant lower quantification of genotypes 1 to 3 was detected. |
| Starting Page | 1 |
| Ending Page | 6 |
| Page Count | 6 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://jcm.asm.org/content/44/3/729.full.pdf |
| PubMed reference number | 16517847v1 |
| Volume Number | 44 |
| Issue Number | 3 |
| Journal | Journal of clinical microbiology |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Branched DNA Signal Amplification Assay Cefotiam Chemical and Drug Induced Liver Injury Genotype HCV viral load Hantavirus Infections Hepatitis C virus Hepatitis C, Chronic Hermanski-Pudlak Syndrome Patients Population Parameter Quantitation RNA RNA, Viral Reverse Transcription |
| Content Type | Text |
| Resource Type | Article |
