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Real-time time-domain fluorescence lifetime imaging including single-shot acquisition with a segmented optical image intensifier
| Content Provider | Semantic Scholar |
|---|---|
| Author | Elson, Daniel S. Munro, Ian Requejo-Isidro, Jose Mcginty, James Dunsby, Christopher Galletly, Neil P. Stamp, Gordon Neil, M. A. A. Lever, M. John Kellett, Paul A. Dymoke-Bradshaw, A. K. L. Hares, J. D. French, Paul Michael William |
| Copyright Year | 2004 |
| Abstract | High-speed (video-rate) fluorescence lifetime imaging (FLIM) is reported using two different time-domain approaches based on gated optical image intensifier technology. The first approach utilizes a rapidly switchable variable delay generator with sequential image acquisition, while the second employs a novel segmented gated optical imager to acquire lifetime maps in a single shot. Lifetimes are fitted using both a non-linear least-squares fit analysis and the rapid lifetime determination method. Monte Carlo simulations were used to optimize the acquisition parameters and a comparison between theory and experiment is presented. The importance of single-shot imaging to minimize the deleterious impact of sample movements is highlighted. Real-time FLIM movies of multi-well plate samples and tissue autofluorescence are presented. |
| Starting Page | 180 |
| Ending Page | 180 |
| Page Count | 1 |
| File Format | PDF HTM / HTML |
| DOI | 10.1088/1367-2630/6/1/180 |
| Volume Number | 6 |
| Alternate Webpage(s) | https://spiral.imperial.ac.uk:8443/bitstream/10044/1/578/1/Elson_4-channel_NJP.pdf |
| Alternate Webpage(s) | https://doi.org/10.1088/1367-2630%2F6%2F1%2F180 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
