NDLI: Micropropagation of an Endangered Medicinal Herb Ocimum Citriodorum Vis

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Micropropagation of an Endangered Medicinal Herb Ocimum Citriodorum Vis

Content Provider	Semantic Scholar
Author	Tripathi, Anamika Abbas, Nazia Shahana Nigam, Amrita
Copyright Year	2014
Abstract	An efficient protocol has been developed for rapid micropropagation of Ocimum citriodorum Vis., an endangered medicinal herb. The cotyledons were excised from the in vitro germinating seedlings and used as explants for the present study. The explants yielded the highest frequency of 87.49% shoot regeneration with an average shoot length of 4.98 cm on Murashige and Skoog (MS) medium supplemented with 1 mg l 6benzylamino purine (BAP) + 0.1 mg l napthalene acetic acid (NAA) + 500 mg l casein hydrolysate (CH) + 25 mg l adenine sulphate (AS). Alteration from the optimal concentration of BAP resulted in the formation of callus. Regenerated microshoots were separated and rooted on MS medium containing NAA (0.5 mg l). Well-developed complete plantlets were transferred onto plastic cups containing sterile soil and humus (1:1). Subsequently the acclimatized plantlets were successfully grown in garden. The regenerated plants were morphologically identical and exhibited similar growth characteristics as compared to the donor plants. Cytological studies of the regenerants revealed no change in chromosome numbers. Thus, regeneration protocol demonstrated in the present study provides a basis for the germplasm conservation and investigation of its medicinally active constituents.
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://jpds.co.in/wp-content/uploads/2019/07/02.AnamikaTripathi1-N.-S.pdf
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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