First Molecular Characterization of Feline Immunodeficiency Virus in Domestic Cats from Mainland China

Content Provider	Semantic Scholar
Author	Zhang, Jilei Wang, Liang Li, Jing Kelly, Patrick Price, Stuart Wang, Chengming
Copyright Year	2017
Abstract	The feline immunodeficiency virus (FIV) is a retrovirus of the Lentivirus genus that was initially isolated from a colony of domestic cats in California in 1986 and has now been recognized as a common feline pathogen worldwide. To date, there is only one recent serology-based report on FIV in mainland China which was published in 2016. We designed this study to investigate the molecular prevalence and diversity of feline immunodeficiency virus (FIV) in domestic cats from mainland China. We studied the prevalence of FIV in whole blood samples of 615 domestic cats in five cities (Beijing, Guangzhou, Nanjing, Shanghai and Yangzhou) of mainland China and examined them using FRET-PCR (Fluorescence Resonance Energy Transfer-Polymerase Chain Reaction) and regular PCRs for the gag and env genes. Overall, 1.3% (8/615) of the cats were positive for provirus DNA with nucleotide analysis using PCRs for the gag and env sequences showing the cats were infected with FIV subtype A. This is the first molecular characterization of FIV in mainland China and the first description of subtype A in continental Asia.
File Format	PDF HTM / HTML
DOI	10.1371/journal.pone.0169739
PubMed reference number	28107367
Journal	Medline
Volume Number	12
Alternate Webpage(s)	https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0169739&type=printable
Alternate Webpage(s)	https://doi.org/10.1371/journal.pone.0169739
Journal	PloS one
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article