NDLI: Endothelial progenitor cell differentiation using cryopreserved, umbilical cord blood-derived mononuclear cells

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Endothelial progenitor cell differentiation using cryopreserved, umbilical cord blood-derived mononuclear cells

Content Provider	Semantic Scholar
Author	Jang, Jun-Ho Kim, Sun-Kyung Choi, Jeong-Eun Kim, Young-Jin Lee, Hyun-Woo Kang, Seok-Yun Park, Joon-Seong Choi, Jin-Hyuk Lim, Ho-Yeong Kim, Hugh Chul
Copyright Year	2007
Abstract	AbstractAim:To investigate the endothelial differentiation potentiality of umbilical cord blood (UCB), we induced the differentiation of endothelial progenitor cells (EPC) from cryopreserved UCB-derived mononuclear cells (MNC).Methods:MNC from cryopreserved UCB and peripheral blood (PB) were cultured in M199 medium with endothelial cell growth supplements for 14 d. EPC were characterized by RT-PCR, flow cytometry, and immunocytochemistry analysis. The proliferation of differentiated EPC was studied by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay, and vascular endothelial growth factor (VEGF) concentration was measured using an ELIS A kit. Characteristics of UCB-derived EPC were compared with those of PB-derived EPC.Results:A number of round-shaped cells were loosely attached to the bottom after 24 h culture, and numerous spindle-shaped cells began to appear from the round-shaped ones on d 7. Those cells expressed endothelial markers such as, Flt-1/VEGFR-1, ecNOS, VE-cadherin, von Willebrand factor, and secreted VEGF. The patterns of endothelial markers of EPC from PB and UCB did not show striking differences. The results of the proliferation and secretion of VEGF were also similar.Conclusion:We successfully cultured UCB cells stored at -196°C into cells with the quality of endothelial cells. Those EPC could be used for angiogenic therapeutics by activating adjacent endothelial cells and enhancing angiogenesis.
Starting Page	367
Ending Page	374
Page Count	8
File Format	PDF HTM / HTML
DOI	10.1111/j.1745-7254.2007.00519.x
Alternate Webpage(s)	http://www.nature.com/aps/journal/v28/n3/pdf/aps200747a.pdf
PubMed reference number	17302999
Alternate Webpage(s)	https://doi.org/10.1111/j.1745-7254.2007.00519.x
Journal	Medline
Volume Number	28
Journal	Acta Pharmacologica Sinica
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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