NDLI: Molecular identification of virulence genes in clinical Salmonella typhimurium strains using the multiplex- PCR method and their antibiotic resistance profile

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Molecular identification of virulence genes in clinical Salmonella typhimurium strains using the multiplex- PCR method and their antibiotic resistance profile

Content Provider	Semantic Scholar
Author	Arjmand-Asl, Mohammad Amini, Kumarss
Copyright Year	2016
Abstract	Background: Salmonella as an enteric, gram negative, facultative anaerobic, obligate parasite causes food poisoning in human. The aim of present study was to identify the virulence genes stn, sopB, slyA, spvc and Phop/Q in Salmonella typhimurium strains isolated from clinical specimens using multiplex PCR method and also the determination of their antibiotic resistance profile. Material and methods: In this cross-sectional study during a 12 month period (April to March 2015) a total of 60 Salmonella typhimurium isolates were collected from Shariati hospital (Tehran, Iran). After the identification of strains, the antimicrobial susceptibility test was done using the disk diffusion agar test on Müller-Hinton agar media according to the Clinical and laboratory standards institute (CLSI) guidelines. Then the presence of virulence genes (e.g. stn, sopB, slyA, spvc and Phop/Q ) were identified using multiplex PCR method. Results: These findings obtained from isolates showed the highest sensitivity to the Imipenem, Gentamicin, and Trimethoprim antibiotics . Distribution analysis of virulence for genes slyA, stn, sopb, Phop/Q showed the frequency of 23.3%, 30%, 3.33%5 and 43.3%, respectively. The spvC gene is not seen in any isolates. Conclusion: The results of this study indicate that the prevalence of virulence genes in clinical Salmonella Typhimurium isolates can serve as an alarm for the prevalence of these genes to the other Salmonella serotypes. As the Salmonella virulence genes are located on the Salmonella pathogenicity islands, so the high prevalence of these genes can help to the dissemination of islands among the Salmonella strains.
Starting Page	376
Ending Page	382
Page Count	7
File Format	PDF HTM / HTML
Volume Number	20
Alternate Webpage(s)	http://feyz.kaums.ac.ir/article-1-3139-fa.pdf
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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