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THE MONOMERIC FORM OF PHOTOSYSTEM I REACTION CENTER OF THE THERMOPHILIC CYANOBACTERIUM Mastigocladus laminosus : RECENT PROGRESS IN CRYSTALLIZATION OF THE COMPLEX
| Content Provider | Semantic Scholar |
|---|---|
| Author | Gilon, Tamar Shaanan, Boaz Nechushtai, Rachel |
| Copyright Year | 2014 |
| Abstract | Preface: The research described is being performed in the Laboratory of Structural Biology at The Hebrew University of Jerusalem. The laboratory was established last year as The Israel National Center for Structural Biology. Different scientific disciplines, that complement each other, are represented in the center: theoretical and computational chemistry, molecular biology and biochemistry, organic synthetic chemistry and X-ray crystallography. The core of the laboratory includes the groups of Prof. Ron Elber-a computational biochemist; Prof. Chalm Gilon=-a bio-organic chemist; Prof. Rachel Nechushtai-a biochemist and molecular biologist and of the crystallographers-Dr. Gil Shoham and Prof. Boaz Shaanan, the Director of the center. The presence of and interactions between the members of the five different departments promote a dynamic research environment in which scientific problems in the forefront of modern biological science are addressed. Among these are the structure-function of chaperons, of enzymes and of several membrane proteins; the dynamics of proteins that participate in the breathing process (like hemoglobin and myoglobin); carbohydrate-protein interactions (Iectins), and others. The X-ray equipment in the laboratory was all purchased through the Rigaku Corporation, Japan. The authors would like to acknowledge the support of the corporation and of their Israeli representative, General Engineers LTD., during the establishment of the center, and look forward to continuing cooperation in the future. The crystallographic equipment includes two rotating-anode X-ray generators (RU-300), a precession camera and an imaging-plate area detector (R-AXIS IIC). The computational equipment consists of two VAX-stations (DEC-3100/38; DEC-31 00/76) and three Silicon Graphics workstations (Personal Iris 25GT, Power-240GTXB and Iris-Elan). The establishment of the center was facilitated by the described project, i.e., the elucidation of the three dimensional structure of the photosystem I reaction center (PSI-RC), one of the two photochemically active complexes present in the thylakoid membranes of oxygenic photosynthetic organisms (higher plants, green algae and cyanobacteria). The present article describes the recent progress in the crystallization of the monomeric form of PSI-RC, and preliminary crystallographic characterization of the newly obtained crystals. Structural Biology Laboratory, Faculty of Science and Mathematics, The Hebrew University of Jerusalem, Jerusalem 91904, ISRAEL |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.rigaku.com/downloads/journal/Vol10.1.1993/rj10-1%20p14-19.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
