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Secondary structure estimation of recombinant psbH, encoding a photosynthetic membrane protein of cyanobacterium Synechocystis sp. PCC 6803
| Content Provider | Semantic Scholar |
|---|---|
| Author | Štys, Dalibor Schöfberger, Wolfgang Halbhuber, Zbyněk Ristvejová, Jaroslava Muller, Nicholas Z. Ettrich, Rüdiger H. |
| Copyright Year | 2005 |
| Abstract | The PsbH protein of cyanobacterium Synechocystis sp. PCC 6803 was expressed as a fusion protein with glutathione-S transferase (GST) in E. coli grown on a mineral medium enriched in 15N isotope. After enzymatic cleavage of the fusion protein, the 1H-15N-HSQC spectrum of PsbH protein in presence of the detergent β-D-octyl-glucopyranoside (OG) was recorded on a Bruker DRX 500 MHz NMR spectrometer equipped with a 5 mm TXI cryoprobe to enhance the sensitivity and resolution. Non-labelled protein was used for secondary structure estimation by deconvolution from circular dichroism (CD) spectra. Experimental results were compared with our results from a structural model of PsbH using a restraint-based comparative modelling approach combined with molecular dynamics and energetic modelling. We found that PsbH shows 34–38% α-helical structure (Thr36-Ser60), a maximum of around 15% of β-sheet, and 12–19% of β-turn. |
| Starting Page | 421 |
| Ending Page | 424 |
| Page Count | 4 |
| File Format | PDF HTM / HTML |
| DOI | 10.1007/s11099-005-0067-1 |
| Volume Number | 43 |
| Alternate Webpage(s) | https://ps.ueb.cas.cz/pdfs/phs/2005/03/14.pdf |
| Alternate Webpage(s) | https://doi.org/10.1007/s11099-005-0067-1 |
| Journal | Photosynthetica |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
