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Integrated Fluorescence-labeling and Polyacrylamide Gel Electrophoresis for Analysis of Protein Isoforms
| Content Provider | Semantic Scholar |
|---|---|
| Author | Apori, Akwasi Asare Herr, Amy E. |
| Copyright Year | 2008 |
| Abstract | We present a new native protein analysis format that automates and integrates all assay steps required for fluorescence-based isoform analysis. Our assay combines salient features of on-chip PAGE (polyacrylamide gel electrophoresis) with wellcontrolled protein labeling within an on-chip 'nanoreactor' defined by a polyacrylamide size-exclusion membrane. The method uses efficient, addressable electrophoretic transport to seamlessly integrate: native protein labeling, background signal reduction, protein enrichment, and native PAGE protein analysis. Results demonstrate native PAGE separations of protein isoforms in less than 2 minutes after protein enrichment and labeling at the membrane. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.rsc.org/binaries/loc/2008/pdfs/papers/006_1009.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
