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Fluorescent hard-sphere polymer colloids for confocal microscopy
| Content Provider | Semantic Scholar |
|---|---|
| Author | Campbell, Andrew I. Bartlett, Paul |
| Copyright Year | 2002 |
| Abstract | We describe a single-stage synthesis of fluorescent hard-sphere polymer colloids. Monodisperse sterically stabilized poly(methyl methacrylate) spheres were prepared by a dispersion copolymerization of methyl methacrylate and methacrylic acid in the presence of a nonpolymerizable dye. The PMMA colloids are intensely fluorescent and when dispersed in hydrocarbons interact as classical hard spheres. At high concentrations they rapidly nucleate highly ordered three-dimensional colloidal crystals. Four different fluorophores were investigated to minimize the degree of fluorescence photobleaching at λ = 488 nm. The dye, 1,1 - -dioctadecyl-3,3,3 - ,3 - tetramethylindocarbocyanine perchlorate (DiIC18), was observed to photobleach at a significantly slower rate than existing materials (Bosma et al., J. Colloid Interface Sci. 245, 292 (2002); Jardine and Bartlett, Colloid Surf. A, in press). We show the usefulness of the resulting DiIC18 particles for confocal investigations of colloidal phenomena. C - 2002 Elsevier Science (USA) |
| Starting Page | 325 |
| Ending Page | 330 |
| Page Count | 6 |
| File Format | PDF HTM / HTML |
| DOI | 10.1006/jcis.2002.8669 |
| Volume Number | 256 |
| Alternate Webpage(s) | http://www.chm.bris.ac.uk/pt/bartlett/pdf/2320b.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
