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Avaliação do plasma rico em plaquetas na proliferação celular: estudo in vitro
| Content Provider | Semantic Scholar |
|---|---|
| Author | Filho, José Scarso |
| Copyright Year | 2002 |
| Abstract | SCARSO FILHO, J. Evaluation of the platelet-rich plasma in the cellular proliferation: an in vitro study. 2002. Thesis (Doctorate of Dentistry), Federal University of Santa Catarina. Platelet-rich plasma (PRP) is considered an accelerator that acts in the tissue repair. A number of clinical investigations substantiate their use, being reported several ways of obtaining, activation and gelation, without establishing their correct concentration and proliferative effects on in vitro cells. This study evaluates the action of three types of PRPs: the pure, that is inside the bag of the platelet concentrate; the activated, that is a mixture of the pure with 10% chloride of calcium; and the gelated, which is an activated and incorporated mixture of particulate bone, after the time of gelation. These types of PRPs were applied on the proliferation of in vitro cells of NIH/3T3 fibroblastic lineage. To develop this methodology, the platelet process was accomplished using a bottle of cell culture, creating cells of the same lineage, where the cytotoxic and proliferative experiment was performed. For cytotoxicity, it was accomplished the protein dosage of 10 bags of PRPs to establish the quantity of stimulus and after several dilution tests and application on in vitro cells was chosen the concentration of 10 ng. For proliferation analyses, the cells previously obtained of the same lineage, were stimulated with 10 ng of PRP, and the control group by the action of 2% and 5 % of SFB. The proliferation was determined by the incorporation of tritiated thymidine in the interval of 20 hours. It was concluded that cellular proliferation stimulated by PRP in the concentration of 10 ng was significant, being more expressive the group of activated PRP. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://repositorio.ufsc.br/xmlui/bitstream/handle/123456789/83737/188845.pdf?isAllowed=y&sequence=1 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
