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IGF-II promoter specific methylation and expression in epithelial ovarian cancer and their associations with disease characteristics.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Qian, Biyun Katsaros, Dionyssios Lu, Lingeng Canuto, Emilie Marion Benedetto, Chiara Beeghly-Fadiel, Alicia Yu, Herbert |
| Copyright Year | 2011 |
| Abstract | High expression of insulin-like growth factor-II (IGF-II) in epithelial ovarian cancer is associated with aggressive disease and poor prognosis. IGF-II transcription is initiated from multiple promoters. Promoter-specific expression is regulated by DNA methylation, which is often dysregulated in cancer. Here, the effects of promoter-specific methylation on IGF-II expression are investigated in ovarian cancer. Fresh tumor samples were collected from 211 patients for analyses of IGF-II promoter methylation using methylation-specific PCR, and of promoter-specific expression of IGF-II mRNA with qRT-PCR, as well as tissue levels of IGF-II peptide with an ELISA. Cox regression analysis was performed to assess IGF-II methylation and expression in association with progression-free and overall survival. DNA methylation was high in IGF-II promoters 2 (P2, 64.2%) and 3 (P3, 52.1%) and low in promoter 4 (P4, 9.8%). High methylation was associated with low mRNA expression in a promoter-specific manner. P3 methylation and expression appeared to be critical in ovarian cancer compared to other promoters. While methylation in an individual promoter was not associated with the disease, a methylation pattern involving P2 and P3 was significantly different among patients with distinct tumor grade, debulking results, residual tumor size and treatment response. The methylation pattern was also associated with disease progression. The study suggests that DNA methylation regulates IGF-II promoter-specific expression in ovarian cancer and the regulation may play a role in disease progression. Assessing methylation patterns in IGF-II promoters may have clinical implications. |
| Starting Page | 63 |
| Ending Page | 82 |
| Page Count | 20 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://www.spandidos-publications.com/or/25/1/203/download |
| PubMed reference number | 21109978v1 |
| Volume Number | 25 |
| Issue Number | 1 |
| Journal | Oncology reports |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Enzyme-Linked Immunosorbent Assay Epithelial ovarian cancer Growth Factor Malignant neoplasm of ovary Mental association Neoplasms Patients Progressive Disease Protein methylation Residual Tumor Somatomedins Tumor Debulking ovarian neoplasm promoter |
| Content Type | Text |
| Resource Type | Article |
