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Characterization of heteropolymeric hexosaminidase A in human X mouse hybrid cells.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Chern, Jin-Ching Beutler, Ernest Kuhl, Wanda Gilbert, Freeman Mellman, William J. Croce, Carlo M. |
| Copyright Year | 1976 |
| Abstract | Expression of heteropolymeric hexosaminidase A activity is reported in a human X mouse hybrid cell line that contains an X/15 translocation chromosome but lacks human chromosome 5 and has no detectable human hexosaminidase B activity. (Hexosaminidase is beta-N-acetylglucosaminidase; EC 3.2.1.30; 2-acetamido-2-deoxy-beta-D-glucoside acetamidodeoxyglucohydrolase.) That the "hexosaminidase A" enzyme of these cells contains the human hexosaminidase-alpha subunit and not the human hexosaminidase-beta subunit is indicated by the cells' reactions to specific antisera prepared against the alpha subunit and against the beta subunit. Our results indicate that the "hexosaminidase A" activity in this hybrid cell line is the expression of a hybrid molecule composed of human hexosaminidase-alpha subunit and a mouse hexosaminidase subunit. These results are consistent with the hypothesis that the human hexosaminidase A enzyme is formed from alpha and beta subunits coded for by genes on chromosomes 15 and 5, respectively. |
| File Format | PDF HTM / HTML |
| DOI | 10.1073/pnas.73.10.3637 |
| PubMed reference number | 62363 |
| Journal | Medline |
| Volume Number | 73 |
| Issue Number | 10 |
| Alternate Webpage(s) | http://www.pnas.org/content/73/10/3637.full.pdf |
| Alternate Webpage(s) | https://doi.org/10.1073/pnas.73.10.3637 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
