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Streptomyces hygroscopicus PACCH 24 ท่ีสร้างเอนไซมไ์คติเนส Biocontrol of Sclerotium rolfsii root and stem rot disease of chili using chitinase-producing actinomycetes , Streptomyces hygroscopicus PACCH 24
| Content Provider | Semantic Scholar |
|---|---|
| Copyright Year | 2012 |
| Abstract | Rhizoshere-associated soils from Ubon Ratchathani and Srisaket provience were collected for isolation of actinomycetes using selective media. Two hundred and eighty three isolates were obtained by using enrichment media for isolation of chitinase-producing actinomycetes agar (EMCA agar). All isolates were screened for chitinolytic activity and sixty eight isolates gave significant clear zone on EMCA agar plates. The selected chitinase-producing isolates were assayed for in vitro antagonism towards Sclerotium rolfsii using cornmeal agar (CMA agar) assay procedure and the result showed that thirteen isolates have remarkable inhibiting the growth of the fungus. There วารสารวทิยาศาสตรแ์ละเทคโนโลย ีมหาวทิยาลยัอุบลราชธานี ปีที ่14 ฉบบัที ่1 มกราคม – มนีาคม 2555 11 were PACCH24, PACCH42, PACCH91, PACCH101, PACCH129, PACCH133, PACCH137, PACCH 140, PACCH 224, PACCH 225, PACCH 246, PACCH 247 and PACCH 277, whereas the top five antagonistic actinomycetes were PACCH 277, PACCH129, PACCH225, PACCH24 and PACCH246, respectively. The results indicated that these actinomycetes produced chitinase which catalyzed chitin, resulting in inhibition of S. rolfsii growth. Their abilities to prevent the disease development were tested for in vivo biocontrol assay on young chili seedling in climate room condition. Two out of thirteen candidate antagonists reduced the disease development at 90%. There were PACCH24 and PACCH225 which was different from in vitro assay result. It showed that the ability to inhibit the pathogen in vitro was not related to the disease reduction in vivo. Maximum biomass production of actinomycetes PACCH24 could be produced by using a combination of yeast extract (0.8 g/l) with sucrose (1.0 g/l) as nitrogen source and carbon source, respectively. Morphology characteristic, diaminopimelic acid, sugar analysis, and 16S rDNA sequencing analysis showed that it was Streptomyces hygroscopicus PACCH24. The study indicated a novel chitinolytic actinomycete could be developed as a biological agent including a complement with organic fertilizers in order to control root and stem rot disease and promoting growth of chili. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.ubu.ac.th/web/files_up/08f2013012309374295.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
