NDLI: Characteristics of a water-forming NADH oxidase from Methanobrevibacter smithii, an archaeon in the human gut

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Characteristics of a water-forming NADH oxidase from Methanobrevibacter smithii, an archaeon in the human gut

Content Provider	Semantic Scholar
Author	Yan, Mingguang Yin, Weibing Fang, Xiao Chao Guo, Jianjun Shi
Copyright Year	2016
Abstract	NADH oxidases (NOXs) catalysing the oxidation of NADH to yield NAD+ and H2O, H2O2, or both play an important role in protecting organisms from oxidative stress and maintaining the balance of NAD+/NADH. A gene encoding NOX was identified from Methanobrevibacter smithii (NOX-ms), the predominant archaeon in the human gut ecosystem. Subsequent analyses showed that it is an FAD-containing protein with a subunit molecular mass of 48 kDa. NOX-ms was purified to homogeneity after expression in Escherichia coli NOX-ms catalysed the oxidization of NADH and converted O2 to H2O with an optimal pH of 7.5 and a temperature optimum of approximately 37°C. The Vmax and Km values were 42.6-44.1 unit/mg and 47.8-54.6 μM for NADH. The apparent Vmax and Km for oxygen were 189.5-196.1 unit/mg and 14.6-16.8 μM. The mutation analysis suggests that Cys42 in NOX-ms plays a key role in the four-electron reduction of O2 to H2O. Quantitative reverse transcription-PCR (RT-qPCR) revealed that transcription of NOX-ms was also up-regulated after exposing the cells to oxidative stress and glucose. Finally, the potential of NOX-ms as a target to control colonization of M. smithii and its possible applications are discussed.
Starting Page	227
Ending Page	432
Page Count	206
File Format	PDF HTM / HTML
PubMed reference number	27737924v1
Alternate Webpage(s)	https://doi.org/10.1042/BSR20160357
DOI	10.1042/bsr20160357
Journal	Bioscience reports
Volume Number	36
Language	English
Access Restriction	Open
Subject Keyword	Archaea Flavin-Adenine Dinucleotide Glucose Hydrogen Peroxide Methanobrevibacter smithii Molecular Mass NADH dehydrogenase (ubiquinone) NADH oxidase Nicotinamide adenine dinucleotide (NAD) Oxidative Stress Oxygen Reverse Transcription Water oxidation
Content Type	Text
Resource Type	Article

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