NDLI: In vitro mimicry of essential fatty acid deficiency in human endothelial cells by TNFα impact of ω-3 versus ω-6 fatty acids

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In vitro mimicry of essential fatty acid deficiency in human endothelial cells by TNFα impact of ω-3 versus ω-6 fatty acids

Content Provider	Semantic Scholar
Author	Mayer, Konstantin Schmidt, Reinhold Ernst Muhly-Reinholz, Marion Bögeholz, Tina Gokorsch, Stephanie Grimminger, Friedrich Seeger, Werner
Copyright Year	2002
Abstract	Severe endothelial abnormalities are a promi- nent feature in sepsis with cytokines such as tumor necrosis factor (TNF) - being implicated in the pathogenesis. As mimic to inflammation, human umbilical vascular endothe- lial cells (HUVEC) were incubated with TNFfor 22 h, in the absence or presence of the - -6 fatty acid (FA), arachi- donic acid (AA), or the alternative - -3 FA eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). TNFcaused marked alterations in the PUFA profile and long chain PUFA content of total phospholipids (PL) decreased. In contrast, there was a compensatory increase in mead acid (MA, 20:3( - -9)), the hallmark acid of the essential fatty acid deficiency (EFAD) syndrome. Corresponding changes were noted in phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, and phosphatidylinositol, but not in the sphingomyelin fraction. Supplementation with AA, EPA, or DHA markedly increased the respective FA contents in the PL pools, suppressed the increase in MA, and resulted in a shift either toward further predominance of - -6 or pre- dominance of - -3 FA. We conclude that short-term TNFincubation of HUVEC causes an EFAD state hitherto only described for long-term malnutrition, and that endot- helial cells are susceptible to differential influence by - -3 versus - -6 FA supplementation under these conditions. — Mayer, K., R. Schmidt, M. Muhly-Reinholz, T. Bogeholz, S. Gokorsch, F. Grimminger, and W. Seeger. In vitro mimicry of essential fatty acid deficiency in human endothelial cells by TNFimpact of - -3 versus - -6 fatty acids. J. Lipid Res. 2002. 43: 944-951.
Starting Page	944
Ending Page	951
Page Count	8
File Format	PDF HTM / HTML
Volume Number	43
Alternate Webpage(s)	http://www.jlr.org/content/43/6/944.full.pdf
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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