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Inhibition of CD34+ cell migration by matrix metalloproteinase-2 during acute myocardial ischemia, counteracted by ischemic preconditioning
| Content Provider | Semantic Scholar |
|---|---|
| Author | Lukovic, Dominika Zlabinger, Katrin Gugerell, Alfred Spannbauer, Andreas Pavo, Noemi Mandic, Ljubica Weidenauer, Denise T. Kastl, Stefan Peter Kaun, Christoph Pósa, Anikó Litschauer, Inna Sabdyusheva Winkler, Jochen Gyöngyösi, Mariann |
| Copyright Year | 2016 |
| Abstract | Background. Mobilization of bone marrow-origin CD34+ cells was investigated 3 days (3d) after acute myocardial infarction (AMI) with/without ischemic preconditioning (IP) in relation to stromal-derived factor-1 (SDF-1α)/ chemokine receptor type 4 (CXCR4) axis, to search for possible mechanisms behind insufficient cardiac repair in the first days post-AMI. Methods. Closed-chest reperfused AMI was performed by percutaneous balloon occlusion of the mid-left anterior descending (LAD) coronary artery for 90min, followed by reperfusion in pigs. Animals were randomized to receive either IP initiated by 3x5min cycles of re-occlusion/re-flow prior to AMI (n=6) or control AMI (n=12). Blood samples were collected at baseline, 3d post-AMI, and at 1-month follow-up to analyse chemokines and mobilized CD34+ cells. To investigate the effect of acute hypoxia, SDF-1α and matrix metalloproteinase (MMP)-2 in vitro were assessed, and a migration assay of CD34+ cells toward cardiomyocytes was performed. Results. Reperfused AMI induced significant mobilisation of CD34+ cells (baseline: 260±75 vs. 3d: 668±180; P<0.001) and secretion of MMP-2 (baseline: 291.83±53.40 vs. 3d: 369.64±72.89; P=0.011) into plasma, without affecting the SDF-1α concentration. IP led to the inhibition of MMP-2 (IP: 165.67±47.99 vs. AMI: 369.64±72.89; P=0.004) 3d post-AMI, accompanied by increased release of SDF-1α (baseline: 23.80±12.36 vs. 3d: 45.29±11.31; P=0.05) and CXCR4 (baseline: 0.59±0.16 vs. 3d: 2.06±1.42; P=0.034), with a parallel higher level of mobilisation of CD34+ cells (IP: 881±126 vs. AMI: 668±180; P=0.026), compared to non-conditioned AMI. In vitro, CD34+ cell migration toward cardiomyocytes was enhanced by SDF-1α, which was completely abolished by 90min hypoxia and co-incubation with MMP-2. Conclusions. Non-conditioned AMI induces MMP-2 release, hampering the ischemia-induced increase in SDF-1α and CXCR4 by cleaving the SDF-1α/CXCR4 axis, with diminished mobilization of the angiogenic CD34+ cells. IP might influence CD34+ cell mobilization via inhibition of MMP-2. |
| Starting Page | 539 |
| Ending Page | 552 |
| Page Count | 14 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://f1000researchdata.s3.amazonaws.com/manuscripts/11302/c760ea72-191e-4817-aff1-8dba85093922_9957_-_dominika_lukovic__v2.pdf?doi=10.12688/f1000research.9957.2 |
| Alternate Webpage(s) | https://f1000researchdata.s3.amazonaws.com/manuscripts/10732/2a731d95-fcd7-4ab1-81bc-3a660ca0eb63_9957_-_dominika_lukovic.pdf?doi=10.12688/f1000research.9957.1 |
| Alternate Webpage(s) | https://f1000researchdata.s3.amazonaws.com/manuscripts/11610/111b8d0b-0dd2-4990-a135-579a99fb26aa_9957_-_dominika_lukovic_v3.pdf?doi=10.12688/f1000research.9957.3 |
| PubMed reference number | 5321121 |
| Alternate Webpage(s) | https://doi.org/10.12688/f1000research.9957.2 |
| DOI | 10.12688/f1000research.9957.2 |
| Journal | F1000Research |
| Volume Number | 5 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Acute myocardial infarction Angiogenic Process Anterior descending branch of left coronary artery Axis vertebra Balloon Occlusion Bone Marrow Cell Migration Assays Hypoxia Ischemic Preconditioning Matrix Metalloproteinase 2 Matrix Metalloproteinases Metalloproteases Myocytes, Cardiac Reperfusion Therapy chemokine receptor |
| Content Type | Text |
| Resource Type | Article |
