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Endophytic colonization and in planta nitrogen fixation by a diazotrophic Serratia sp. in rice.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Sandhiya, G. S. Sugitha, T. C. Kumari Balachandar, Danajeyan Kumar, K. S. Manoj |
| Copyright Year | 2005 |
| Abstract | Nitrogen fixing endophytic Serratia sp. was isolated from rice and characterized. Re-colonization ability of Serratia sp. in the rice seedlings as endophyte was studied under laboratory condition. For detecting the re-colonization potential in the rice seedlings, Serratia sp. was marked with reporter genes (egfp and Kmr) using transposon mutagenesis. The conjugants were screened for re-colonization ability and presence of nif genes using PCR. Further, the influence of flavonoids and growth hormones on the endophytic colonization and in planta nitrogen fixation of Serratia was also investigated. The flavonoids, quercetin (3 microg/ml) and diadzein (2 microg/ml) significantly increased the re-colonization ability of the endophytic Serratia, whereas the growth hormones like IAA and NAA (5 microg/ml) reduced the endophytic colonization ability of Serratia sp. Similarly, the in planta nitrogen fixation by Serratia sp. in rice was significantly increased due to flavonoids. The inoculation of endophytic diazotrophs increased the plant biomass and biochemical constituents. |
| Starting Page | 422 |
| Ending Page | 425 |
| Page Count | 4 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://nopr.niscair.res.in/bitstream/123456789/23240/1/IJEB%2043(9)%20802-807.pdf |
| PubMed reference number | 16187531v1 |
| Volume Number | 43 |
| Issue Number | 9 |
| Journal | Indian journal of experimental biology |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Biomass Blood urea nitrogen measurement Endophytes Flavonoids Genes, Reporter Growth Hormone Jumping Genes N-acetylaspartate Nitrogen Fixation Phytophthora inflata Quercetin Seedlings Serratia Infections Sole of Foot daidzein |
| Content Type | Text |
| Resource Type | Article |
