NDLI: Genome Sequence Analysis and Characterization of Recombinant Enzymes from the Thermoacidophilic Archaeon Picrophilus torridus

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Genome Sequence Analysis and Characterization of Recombinant Enzymes from the Thermoacidophilic Archaeon Picrophilus torridus

Content Provider	Semantic Scholar
Author	Angelov, Angel
Copyright Year	2004
Abstract	Picrophilus torridus is a thermo- and acidophilic archaeon which grows optimally at 60°C and pH 0.7 and thus represents the most acidophilic organism known so far. In the frame of the current work the complete genome sequence of P. torridus was determined followed by analysis of the identified genes and the possible metabolic pathways which they possibly encode. The information obtained from the genome was further interpreted with respect to the unique characteristics of the organism. In particular, it was anticipated that the genome sequence of P.torridus would allow a more intense examination of the evolutionary relations among organisms that share similar extreme growth conditions under the special consideration of lateral gene transfer. In order to further investigate the adaptation mechanisms allowing the organism to survive at low pH values, several P. torridus open reading frames were selected for heterologous expression. Standard (E. coli) as well as alternative (S. solfataricus and S. cerevisiae) expression systems were used. Two recombinant enzymes were produced and purified, i.e., α-glucosidase (MalP) and glucose-1-dehydrogenase (GdhA), and biochemically characterised.
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://ediss.uni-goettingen.de/bitstream/handle/11858/00-1735-0000-0006-ACD9-E/angelov.pdf;jsessionid=F055D66CF9074254B80250C1616070A6?sequence=1
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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