NDLI: Nanobiomedicine Aminosilane Functionalization and Cytotoxicity Effects of Upconversion Nanoparticles Y 2 O 3 and Gd 2 O 3 Co-doped with Yb 3 + and Er 3 +

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Nanobiomedicine Aminosilane Functionalization and Cytotoxicity Effects of Upconversion Nanoparticles Y 2 O 3 and Gd 2 O 3 Co-doped with Yb 3 + and Er 3 +

Content Provider	Semantic Scholar
Author	Chavez, Dalia Holanda Juárez-Moreno, Karla Hirata, Gustavo A.
Copyright Year	2016
Abstract	In this study, luminescent upconversion nanoparticles (UCNPs) Y2O3 and Gd2O3 co-doped with Yb3+ and Er3+ were prepared by the sol-gel method (SG). These NPs are able to absorb near infrared photons and upconvert them into visible radiation with a direct application in bioimaging, as an important tool to diagnose and visualize cancer cells. The UCNPs were coated with a thin silica shell and functionalized with amino groups for further folic acid conjugation to allow their interaction with folate ligands on the cell surface. Their physical properties were analysed by Transmission Electron Microscopy (TEM), Fourier trans‐ form infrared spectroscopy (FTIR) and photoluminescence (PL) measurements. The PL results revealed excellent luminescence properties on all core-shell UCNPs. Cytotox‐ icity experiments with concentrations of bare and amino‐ silane coated/functionalized UCNPs between 0.001 μg/mL to 1 μg/mL were tested on two different cell lines from human cervix carcinoma (HeLa) and human colorectal adenocarcinoma (DLD-1) with a colorimetric assay based on the reduction of MTT reagent (methy-134-thiazolylte‐ trazolium). The assays show that some concentrations of bare UCNPs were cytotoxic for cervical adenocarcinoma cells (HeLa); however, for human colorectal adenocarcino‐ ma all UCNPs are non-cytotoxic. After UCNPs functional‐ ization with silica-aminosilane (APTES/TEOS), all of the nanoparticles tested were found to be non-cytotoxic for both cell lines. The UCNPs functionalized in this work can be further conjugated with specific ligands and used as biolabels for detection of cancer cells.
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://hrcak.srce.hr/file/232419
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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