Early interleukin 12 production by macrophages in response to mycobacterial infection depends on interferon gamma and tumor necrosis factor alpha

Content Provider	Semantic Scholar
Author	Flesch, Inge E. A. Hess, J. H. Huang, Shuang Aguet, Michel Rothe, Joachim G. Bluethmann, Horst Kaufmann, Stefan H. E.
Copyright Year	1995
Abstract	Interleukin 12 (IL-12) produced by macrophages immediately after infection is considered essential for activation of a protective immune response against intracellular pathogens. In the murine Mycobacterium bovis Bacillus Calmette-Guérin (BCG) model we assessed whether early IL-12 production by macrophages depends on other cytokines. In vitro, murine bone marrow-derived macrophages produced IL-12 after infection with viable M. bovis BCG or stimulation with LPS, however, priming with recombinant interferon gamma (rIFN-gamma) was necessary. In addition, IL-12 production by these macrophages was blocked by specific anti-tumor necrosis factor alpha (TNF-alpha) antiserum. Macrophages from gene deletion mutant mice lacking either the IFN-gamma receptor or the TNF receptor 1 (p55) failed to produce IL-12 in vitro after stimulation with rIFN-gamma and mycobacterial infection. In vivo, IL-12 production was induced in spleens of immunocompetent mice early during M. bovis BCG infection but not in those of mutant mice lacking the receptors for IFN-gamma or TNF. Our results show that IL-12 production by macrophages in response to mycobacterial infection depends on IFN-gamma and TNF. Hence, IL-12 is not the first cytokine produced in mycobacterial infections.
Starting Page	1615
Ending Page	1621
Page Count	7
File Format	PDF HTM / HTML
PubMed reference number	7722441v1
Volume Number	181
Journal	The Journal of experimental medicine
Alternate Webpage(s)	https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/09/94/je18151615.PMC2192016.pdf
Alternate Webpage(s)	http://ftp.ncbi.nlm.nih.gov/pub/pmc/09/94/je18151615.PMC2192016.pdf
Language	English
Access Restriction	Open
Subject Keyword	BCG Vaccine Biomarkers, Tumor Bone Marrow ELECTROSURGICAL DEVICE Gene Deletion Immune Sera Immunocompetent Interferon Alfa-2b Interferon Gamma Receptor Complex Interferon Type II Interleukin Receptor Common gamma Subunit Mycobacterium Infections Mycobacterium bovis Neoplasms Priming Exercise Recombinant Interferon-gamma Recombinant Interleukins Spleen cytokine macrophage tumor necrosis
Content Type	Text
Resource Type	Article