NDLI: Augmentation of ouabain sensitivity of rat liver Na/K-ATPase by in vivo adenovirus-mediated expression of the Na/K-ATPase alpha2 subunit.

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Augmentation of ouabain sensitivity of rat liver Na/K-ATPase by in vivo adenovirus-mediated expression of the Na/K-ATPase alpha2 subunit.

Content Provider	Semantic Scholar
Author	Askari, Fred K. Hitomi, Yoshinori Xie, Zheng
Copyright Year	1997
Abstract	These are the first experiments to study the effect of in vivo expression of the Na/K-ATPase alpha2 subunit which serves as a receptor for cardiac glycosides. The alpha2 subunit is not normally expressed in rat liver, so hepatocytes which lack endogenous alpha2 protein are a logical first target to study the effects of alpha2 expression on membrane Na/K-ATPase activity. At 3 days after alpha2 adenovirus vector infusion, Wistar rat livers contained alpha2 DNA, alpha2 mRNA, and alpha2 protein. Rat liver membrane ouabain binding activity and the sensitivity of Na/K-ATPase activity to ouabain significantly increased. Total membrane Na/K-ATPase was regulated at a constant level while expressed alpha2 activity represented 10% of the total active Na/K-ATPase sites in alpha2 transduced rat liver. These studies are the first to establish a paradigm in which an endogenous drug receptor is expressed to alter cellular pharmacologic sensitivity.
Starting Page	5
Ending Page	10
Page Count	6
File Format	PDF HTM / HTML
DOI	10.1016/S0014-5793(97)00087-2
Alternate Webpage(s)	https://deepblue.lib.umich.edu/bitstream/handle/2027.42/117011/feb2s0014579397000872.pdf;jsessionid=C43E52F70D0D30088F221244B144AAF4?sequence=1
PubMed reference number	9094414
Alternate Webpage(s)	https://doi.org/10.1016/S0014-5793%2897%2900087-2
Journal	Medline
Volume Number	405
Issue Number	1
Journal	FEBS letters
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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