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Thermal stress increases oxidative DNA damage in coral cell aggregates
| Content Provider | Semantic Scholar |
|---|---|
| Author | Nesa, Badrun Hidaka, Masayuki |
| Copyright Year | 2009 |
| Abstract | We have established an experimental system to study the response of coral cells to stresses using coral cell aggregates (tissue balls). The objective of this study was to evaluate the use of tissue balls for studies of oxidative DNA damage in corals cells under thermal stress. Tissue balls prepared from dissociated cells of Pavona divaricata were exposed to high temperature (31°C) in the presence or absence of an antioxidant or to normal temperature (25°C). DNA damage of coral cells was investigated using a Comet Assay (alkaline singlecell gel electrophoresis). The comet tail length, which indicates the degree of DNA damage, was significantly longer in coral cells from tissue balls exposed to 31°C than those from the controls (25°C) or those treated at 31°C in the presence of an antioxidant, 10 mM mannitol. The present results suggest that coral cells suffer oxidative DNA damage under thermal stress. It was not clear whether DNA damage occurred in algal cells as algal DNA was retained within the cell wall and the comet tail was not observed. This study also showed that tissue balls provide us with a good experimental system to study the effect of stress and various chemical reagents on coral cells. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://nsuworks.nova.edu/cgi/viewcontent.cgi?article=1000&context=occ_icrs&filename=29&type=additional |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
