Effect of hypercholesterolemia on vascular reactivity in the rabbit. I. Endothelium-dependent and endothelium-independent contractions and relaxations in isolated arteries of control and hypercholesterolemic rabbits.

Content Provider	Semantic Scholar
Author	Verbeuren, Tony J. Jordaens, F. H. Zonnekeyn, L. L. Hove, Cor E. Van Coene, M. C. Herman, Arnold G.
Copyright Year	1986
Abstract	We studied the effects of hypercholesterolemia on vascular responsiveness in different arteries isolated from rabbits: control groups of rabbits and groups receiving the atherogenic diet consisted of eight animals each. In the arteries, 16 weeks of cholesterol-rich (0.3%) diet evoked intimal lesions which were more pronounced than those noted after 8 weeks of hypercholesterolemia; the aortic arch was affected significantly more by the lesions than the abdominal aorta and the pulmonary artery. Segments of the arteries were mounted in organ chambers for isometric tension recording or for measurement of the endothelium-derived relaxant factor. Contractions caused by acetylcholine and prostaglandin F2 alpha were not altered by the hypercholesterolemia; those evoked by serotonin were moderately augmented only in the aortic arch of hypercholesterolemic rabbits. As the degree of intimal lesion formation increased, the contractions to norepinephrine and clonidine were progressively inhibited. The endothelium-independent relaxations to nitroglycerin were inhibited in only the most severely affected arteries; the endothelium-dependent relaxations to acetylcholine and adenosine triphosphate were progressively inhibited as the degree of fatty streak formation augmented. Thus, in the aortic arch, the relaxations to 3 X 10(-6) M acetylcholine, expressed as percent of the initial contraction, decreased from 86.7 +/- 3.3% in control tissues to 16.3 +/- 8.6% in the 16-week hypercholesterolemic vessels; in the abdominal aortas these relaxations averaged 93.5 +/- 2.2% in control vessels and 72.0 +/- 6.9% in the hypercholesterolemic tissues. The acetylcholine-induced release of endothelium-derived relaxant factor from the abdominal aorta was not significantly affected by the hypercholesterolemia. We conclude from these studies that in arteries obtained from hypercholesterolemic rabbits: the contractions caused by serotonergic mechanisms tend to be augmented, while those to alpha-adrenergic activation are decreased, the endothelium-independent relaxations are modified only in the more severely affected arteries, and the endothelium-dependent relaxations are progressively inhibited as the degree of fatty streak formation augments, probably because a step subsequent to the release of endothelium-derived relaxant factor is altered.
Starting Page	1
Ending Page	4
Page Count	4
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Alternate Webpage(s)	http://circres.ahajournals.org/content/circresaha/58/4/552.full.pdf?download=true
Alternate Webpage(s)	http://circres.ahajournals.org/content/circresaha/58/4/552.full.pdf
PubMed reference number	3486053v1
Volume Number	58
Issue Number	4
Journal	Circulation research
Language	English
Access Restriction	Open
Subject Keyword	Abdominal aorta structure Abstract Summary Acetylcholine Adenosine Triphosphate Aortic arch structure Arteries Atherosclerosis Blood Vessel Body tissue Cholesterol Clonidine Coronary Arteriosclerosis Diet, Atherogenic Dinoprost Endothelium Endothelium-Dependent Relaxing Factors Hypercholesterolemia Manuscripts Moderate Response Monkeys Muscle Cells Muscle relaxation phase Muscle, Smooth, Vascular Myocytes, Smooth Muscle Nitric Oxide Nitroglycerin Norepinephrine Old World rabbit Patients Peripheral Vascular Diseases Prostaglandins Pulmonary artery structure Rabbits Scientific Publication Serotonin Smooth muscle (tissue) Tension Vasoconstrictor Agents Vasospasm, Intracranial Water henry relaxation of smooth muscle relaxation of vascular smooth muscle
Content Type	Text
Resource Type	Article