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The Na+-translocating NADH:quinone oxidoreductase (Na+-NQR) from Vibrio cholerae enhances insertion of FeS in overproduced NqrF subunit.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Tao, Minli Fritz, Guenter Steuber, Julia |
| Copyright Year | 2008 |
| Abstract | The Na+-translocating NADH:quinone oxidoreductase (Na+-NQR) from Vibrio cholerae is a membrane-bound, respiratory Na+ pump. Its NqrF subunit contains one FAD and a [2Fe-2S] cluster and catalyzes the initial oxidation of NADH. A soluble variant of NqrF lacking its hydrophobic, N-terminal helix (NqrF') was produced in V. cholerae wild type and nqr deletion strain. Under identical conditions of growth and induction, the yield of NqrF' increased by 30% in the presence of the Na+-NQR. FAD-containing NqrF' species with or without the FeS cluster were observed, indicating that assembly of the FeS center, but not insertion of the flavin cofactor, was limited during overproduction in V. cholerae. A comparison of these distinct NqrF' species with regard to specific NADH dehydrogenase activity, pH dependence of activity and thermal inactivation showed that NqrF' lacking the [2Fe-2S] cluster was less stable, partially unfolded, and therefore prone to proteolytic degradation in V. cholerae. We conclude that the overall yield of NqrF' critically depends on the amount of fully assembled, FeS-containing NqrF' in the V. cholerae host cells. The Na+-NQR is proposed to increase the stability of NqrF' by stimulating the maturation of FeS centers. |
| File Format | PDF HTM / HTML |
| DOI | 10.1016/j.jinorgbio.2008.01.009 |
| PubMed reference number | 18289689 |
| Journal | Medline |
| Volume Number | 102 |
| Issue Number | 5-6 |
| Alternate Webpage(s) | http://www.zora.uzh.ch/2318/2/Tao_J_Inorg_Biochem_2008.pdf |
| Alternate Webpage(s) | https://doi.org/10.1016/j.jinorgbio.2008.01.009 |
| Journal | Journal of inorganic biochemistry |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
