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Strain-specific polymorphisms in Paneth cell α-defensins of C57BL/6 mice and evidence of vestigial myeloid α-defensin pseudogenes.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Shanahan, Michael T. Tanabe, Hiroki Ouellette, André J. |
| Copyright Year | 2011 |
| Abstract | Paneth cells at the base of small intestinal crypts secrete microbicidal α-defensins, termed cryptdins (Crps) in mice, as mediators of innate immunity. Proteomic studies show that five abundant Paneth cell α-defensins in C57BL/6 mice are strain specific in that they have not been identified in other inbred strains of mice. Two C57BL/6-specific peptides are coded for by the Defcr20 and -21 genes evident in the NIH C57BL/6 genome but absent from the Celera mixed-strain assembly, which excludes C57BL/6 data and differs from the NIH build with respect to the organization of the α-defensin gene locus. Conversely, C57BL/6 mice lack the Crp1, -2, -4, and -6 peptides and their corresponding Defcr1, -2, -4, and -6 genes, which are common to several mouse strains, including those of the Celera assembly. In C57BL/6 mice, α-defensin gene diversification appears to have occurred by tandem duplication of a multigene cassette that was not found in the mixed-strain assembly. Both mouse genome assemblies contain conserved α-defensin pseudogenes that are closely related to functional myeloid α-defensin genes in the rat, suggesting that the neutrophil α-defensin defect in mice resulted from progressive gene loss. Given the role of α-defensins in shaping the composition of the enteric microflora, such polymorphisms may influence outcomes in mouse models of disease or infection. |
| File Format | PDF HTM / HTML |
| DOI | 10.1128/IAI.00996-10 |
| PubMed reference number | 21041494 |
| Journal | Medline |
| Volume Number | 79 |
| Issue Number | 1 |
| Alternate Webpage(s) | http://iai.asm.org/content/79/1/459.full.pdf |
| Journal | Infection and immunity |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
