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Three-dimensional imaging and morphometric analysis of alveolar tissue from microfocal X-ray-computed tomography.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Litzlbauer, Horst Detlef Neuhaeuser, Christoph Moell, Alexander Greschus, Susanne Breithecker, Andreas Franke, Folker Ernst Kummer, Wolfgang Rau, Wigbert |
| Copyright Year | 2006 |
| Abstract | We evaluated microfocal X-ray-computed tomography (micro-CT) as a method to visualize lung architecture two and three dimensionally and to obtain morphometric data. Inflated porcine lungs were fixed by formaldehyde ventilation. Tissue samples (8-mm diameter, 10-mm height) were stained with osmium tetroxide, and 400 projection images (1,024 x 1,024 pixel) were obtained. Continuous isometric micro-CT scans (voxel size 9 microm) were acquired to reconstruct two- and three-dimensional images. Tissue samples were sectioned (8-microm thickness) for histological analysis. Alveolar surface density and mean linear intercept were assessed by stereology-based morphometry in micro-CT scans and corresponding histological sections. Furthermore, stereology-based morphometry was compared with morphometric semi-automated micro-CT analysis within the same micro-CT scan. Agreement of methods was assessed by regression and Bland-Altman analysis. Comparing histology with micro-CT, alveolar surface densities (35.4 +/- 2.4 vs. 33.4 +/- 1.9/mm, P < 0.05) showed a correlation (r = 0.72; P = 0.018) with an agreement of 2 +/- 1.6/mm; the mean linear intercept (135.7 +/- 14.5 vs. 135.8 +/- 15 microm) correlated well (r = 0.97; P < 0.0001) with an agreement of -0.1 +/- 3.4 microm. Semi-automated micro-CT analysis resulted in smaller alveolar surface densities (33.4 +/- 1.9 vs. 30.5 +/- 1/mm; P < 0.01) with a correlation (r = 0.70; P = 0.023) and agreement of 2.9 +/- 1.4/mm. Non-destructive micro-CT scanning offers the advantage to visualize the spatial tissue architecture of small lung samples two and three dimensionally. |
| Starting Page | 836 |
| Ending Page | 840 |
| Page Count | 5 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://ajplung.physiology.org/content/ajplung/291/3/L535.full.pdf |
| Alternate Webpage(s) | http://ajplung.physiology.org/content/ajplung/291/3/l535.full.pdf |
| PubMed reference number | 16679382v1 |
| Volume Number | 291 |
| Issue Number | 3 |
| Journal | American journal of physiology. Lung cellular and molecular physiology |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Body tissue Diagnostic radiologic examination Diameter (qualifier value) Formaldehyde Gastroenteritis, Transmissible, of Swine Intercept Substance Morphometric analysis Osmium Tetroxide Plain x-ray Porcine species Pulmonary hypoplasia Respiration Scanning Small Structure of parenchyma of lung Tomography, Spiral Computed X-Ray Computed Tomography density |
| Content Type | Text |
| Resource Type | Article |
