NDLI: Goniothalamin induces apoptosis associated with autophagy activation through MAPK signaling in SK-BR-3 cells.

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Goniothalamin induces apoptosis associated with autophagy activation through MAPK signaling in SK-BR-3 cells.

Content Provider	Semantic Scholar
Author	Innajak, Sukanda Mahabusrakum, Wilawan Watanapokasin, Ramida Yuwadee
Copyright Year	2016
Abstract	Goniothalamin, a plant bioactive styrly-lactone, possesses many biological activities. In the present study, the anticancer effect of goniothalamin on human breast cancer cell line SK-BR-3 was investigated. The results showed that goniothalamin induced nuclear condensation, DNA fragmentation, apoptotic bodies and mitochondrial dysfunction as determined by JC-1 staining. Goniothalamin also increased the Bax/Bcl-2 ratio and expression of cleaved caspase-7, cleaved caspase-9 and cleaved PARP, but decreased Bcl-2 expression. In addition, goniothalamin induced apoptosis via p-JNK1/2 and p-p38 upregulation and inhibited cell survival via p-ERK1/2 and p-Akt downregulation. Notably, goniothalamin induced autophagy through upregulation of Atg7, Atg12-Atg5 conjugation and LC3II. The increased p-p38 and p-JNK1/2 and decreased p-Akt may lead to autophagy induction. Therefore, goniothalamin promoted apoptosis associated with autophagy induction in SK-BR-3 cells through p-p38 and p-JNK1/2 upregulation and p-Akt downregulation. The present study indicated that goniothalamin may be further used as a potential therapeutic candidate or may offer an alternative treatment for breast cancer.
Starting Page	261
Ending Page	261
Page Count	1
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://spandidos-publications.com/or/35/5/2851/download
PubMed reference number	26987063v1
Alternate Webpage(s)	https://doi.org/10.3892/or.2016.4655
DOI	10.3892/or.2016.4655
Journal	Oncology reports
Volume Number	35
Issue Number	5
Language	English
Access Restriction	Open
Subject Keyword	Apoptosis Apoptotic Bodies Autophagy BCL2 gene Breast Cancer Cell Cell Survival Cleaved cell Cultured Cell Line DNA Fragmentation Down-Regulation Human body Lactones Mammary Neoplasms Mitochondrial Diseases Proto-Oncogene Proteins c-akt Up-Regulation (Physiology) caspase-7 caspase-9 goniothalamin romidepsin
Content Type	Text
Resource Type	Article

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