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The yeast splicing factor Prp40p contains functional leucine-rich nuclear export signals that are essential for splicing.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Murphy, Mark W. Olson, Brian L. Siliciano, Paul G. |
| Copyright Year | 2004 |
| Abstract | To investigate the function of the essential U1 snRNP protein Prp40p, we performed a synthetic lethal screen in Saccharomyces cerevisiae. Using an allele of PRP40 that deletes 47 internal residues and causes only a slight growth defect, we identified aphenotypic mutations in three distinct complementation groups that conferred synthetic lethality. The synthetic phenotypes caused by these mutations were suppressed by wild-type copies of CRM1 (XPO1), YNL187w, and SME1, respectively. The strains whose synthetic phenotypes were suppressed by CRM1 contained no mutations in the CRM1 coding sequence or promoter. This indicates that overexpression of CRM1 confers dosage suppression of the synthetic lethality. Interestingly, PRP40 and YNL187w encode proteins with putative leucine-rich nuclear export signal (NES) sequences that fit the consensus sequence recognized by Crm1p. One of Prp40p's two NESs lies within the internal deletion. We demonstrate here that the NES sequences of Prp40p are functional for nuclear export in a leptomycin B-sensitive manner. Furthermore, mutation of these NES sequences confers temperature-sensitive growth and a pre-mRNA splicing defect. Although we do not expect that yeast snRNPs undergo compartmentalized biogenesis like their metazoan counterparts, our results suggest that Prp40p and Ynl187wp contain redundant NESs that aid in an important, Crm1p-mediated nuclear export event. |
| Starting Page | 53 |
| Ending Page | 65 |
| Page Count | 13 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.genetics.org/content/genetics/166/1/53.full.pdf |
| PubMed reference number | 15020406v1 |
| Volume Number | 166 |
| Issue Number | 1 |
| Journal | Genetics |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Contain (action) Copy (object) Deletion Mutation Encode (action) Leucine NES gene Nuclear Export Signals Open Reading Frames Phenotype RNA Splicing Ribonucleoprotein, U1 Small Nuclear XPO1 gene XPO1 wt Allele interferon alfacon-1 leptomycin B negative regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay |
| Content Type | Text |
| Resource Type | Article |
