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Comparison of proliferating cell nuclear antigen to tritiated thymidine as a marker of proliferating hepatocytes in rats.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Foley, Julie Ton, Thai-Vu T. Maronpot, Robert R. Butterworth, Byron E. Goldsworthy, Thomas L. |
| Copyright Year | 1993 |
| Abstract | Proliferating cell nuclear antigen (PCNA), an endogenous nuclear protein, has recently been used to identify replicating cells. PCNA was compared to tritiated thymidine ([3H]-TdR), a reliable and accurate exogenous labeling agent, to ascertain if PCNA gives comparable results for quantitative cell proliferation. Male F344 rats were treated with a single dose of 500 mg/kg 4-acetylaminofluorene (4-AAF), a known liver mitogen. Rats (n = 5) were euthanized and necropsied at 6, 12, 18, 24, 36, 48, 96, or 192 hr after treatment. Two hours before necropsy, rats were pulsed-dosed with [3H]-TdR (2 mCi/kg body weight). Livers were sectioned, autoradiography performed, and labeling indexes (LI), a measurement of the percentage of S-phase hepatocytes, determined. One and a half years after the completion of this study, the archival paraffin blocks of the liver tissue were sectioned and stained for PCNA by an immunohistochemical procedure. Immunocytochemical staining patterns of proliferating cell nuclear antigen antigen expression permitted the recognition of G1, S, G2, M, and quiescent cells. PCNA LI, generated by scoring only cells exhibiting S-phase staining patterns, was compared to the pulse [3H]-TdR LI for each animal. Similar periportal staining patterns of S-phase nuclei were detected by both markers. The [3H]-TdR LI and the PCNA LI exhibited a peak at 24 hr of approximately the same magnitude. However, while the [3H]-TdR LI had returned to near baseline at the 48-hr time point, the PCNA LI remained elevated until the 96-hr time point. This sustained elevation of the PCNA index cannot be explained at this time.(ABSTRACT TRUNCATED AT 250 WORDS) |
| Starting Page | 199 |
| Ending Page | 205 |
| Page Count | 7 |
| File Format | PDF HTM / HTML |
| PubMed reference number | 7912186 |
| Volume Number | 101 |
| Journal | Environmental health perspectives |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | 4-acetylaminofluorene Antigens, Nuclear Autopsy Autoradiography Cell Proliferation Exhibits as Topic Growth Factor In Situ Nick-End Labeling Index Leukemia, B-Cell Liver diseases Liver parenchyma Nuclear Proteins Proliferating Cell Count Proliferating Cell Nuclear Antigen Staining method Thymidine |
| Content Type | Text |
| Resource Type | Article |
