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Laminin deposition is dispensable for vasculogenesis but regulates blood vessel diameter independent of flow.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Jakobsson, Lars Domogatskaya, Anna Tryggvason, K. Edgar, David L. Claesson-Welsh, Lena |
| Copyright Year | 2008 |
| Abstract | Basement membranes (BMs) consisting of laminins, collagens, and heparan sulfate proteoglycans (HSPGs) are vital for proper endothelial cell function, but many aspects of their role in vascular development remain unknown. Here, we demonstrate that vascular structures within differentiating embryoid bodies are wrapped in a BM composed of alpha4- and alpha5-chain laminins, fibronectin, collagen IV, and HSPGs. In sprouting angiogenesis, laminins were produced by stalk cells, as well as the leading tip cell, and deposited along the sprout length, including tip cell filopodia. In embryonic stem cells deficient in laminins, due to lamc1 (laminin gamma1) deletion, vascular development and organization were largely unaffected. However, the frequency of vessels with wide lumens was increased 4-fold. Laminin-deficient vessels were moreover characterized by increased fibronectin levels and enhanced endothelial cell proliferation. We conclude that laminins are dispensable for vascular development but that they regulate lumen formation in the absence of flow and vascular tone. |
| Starting Page | 1 |
| Ending Page | 6 |
| Page Count | 6 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.fasebj.org/content/22/5/1530.full.pdf |
| PubMed reference number | 18073332v1 |
| Volume Number | 22 |
| Issue Number | 5 |
| Journal | FASEB journal : official publication of the Federation of American Societies for Experimental Biology |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Blood Vessel Cell physiology Collagen Deletion Mutation Diameter (qualifier value) Endothelial Cells Eye foreign bodies Glomerular Basement Membrane Heparan Sulfate Proteoglycans Heparitin Sulfate Human body LAMC1 gene Proteoglycan Stem cells Structure of lumen of body system endothelial cell proliferation laminin gamma 1 negative regulation of type IV pilus biogenesis sprouting angiogenesis vasculogenesis |
| Content Type | Text |
| Resource Type | Article |
