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Remodeling of nuclear architecture during the cell cycle in Drosophila embryos.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Johansen, Kristen M. Johansen, Jørgen Baek, Kwan Hyuck Jin, Ye |
| Copyright Year | 1996 |
| Abstract | Little is known about what determines the nuclear matrix or how its reorganization is regulated during mitosis. In this study we report on a monoclonal antibody, mAb2A, which identifies a novel nuclear structure in Drosophila embryos which forms a diffuse meshwork at interphase but which undergoes a striking reorganization into a spindle-like structure during pro- and metaphase. Double labelings with alpha-tubulin and mAb2A antibodies demonstrate that the microtubules of the mitotic apparatus co-localize with this mAb2A labeled structure during metaphase, suggesting it may serve a role in microtubule spindle assembly and/or function during nuclear division. That the mAb2A-labeled nuclear structure is essential for cell division and/or maintenance of nuclear integrity was directly demonstrated by microinjection of mAb2A into early syncytial embryos which resulted in a disintegration of nuclear morphology and perturbation of mitosis. |
| Starting Page | 586 |
| Ending Page | 589 |
| Page Count | 4 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.bb.iastate.edu/~kristen/jcnjo96.pdf |
| PubMed reference number | 8913878v1 |
| Volume Number | 63 |
| Issue Number | 3 |
| Journal | Journal of cellular biochemistry |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Cell Cycle Embryo Interphase Microinjections Microtubules Mitosis Mitotic Metaphase Mitotic Spindle Apparatus Monoclonal Antibodies Nuclear Matrix Nuclear Structure Tracer nuclear division |
| Content Type | Text |
| Resource Type | Article |
