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N-glycan moieties of the crustacean egg yolk protein and their glycosylation sites
| Content Provider | Semantic Scholar |
|---|---|
| Author | Roth, Ziv Parnes, Shmuel Wiel, Simy Sagi, Amir Zmora, Nili Chung, J. Sook Khalaila, Isam |
| Copyright Year | 2009 |
| Abstract | Vitellogenin (Vg) is the precursor of the egg yolk glycoprotein of crustaceans. In the prawn Macrobrachium rosenbergii, Vg is synthesized in the hepatopancreas, secreted to the hemolymph, and taken up by means of receptor-mediated endocytosis into the oocytes. The importance of glycosylation of Vg lies in its putative role in the folding, processing and transport of this protein to the egg yolk and in the fact that the N-glycan moieties could provide a source of carbohydrate during embryogenesis. The present study describes, for the first time, the structure of the glycan moieties and their sites of attachment to the Vg of M. rosenbergii. Bioinformatics analysis revealed seven putative N-glycosylation sites in M. rosenbergii Vg; two of these glycosylation sites are conserved throughout the Vgs of decapod crustaceans from the Pleocyemata suborder (N159 and N660). The glycosylation of six putative sites of M. rosenbergii Vg (N151, N159, N,168N,614N660 and N2300) was confirmed; three of the confirmed glycosylation sites are localized around the N-terminally conserved N-glycosylation site N159. From a theoretical three-dimensional structure, these three N-glycosylated sites N151, N159, and N168 were localized on the surface of the Vg consensus sequence. In addition, an uncommon high mannose N-linked oligosaccharide structure with a glucose cap (Glc1Man9GlcNAc2) was characterized in the secreted Vg. These findings thus make a significant contribution to the structural elucidating of the crustacean Vg glycan moieties, which may shed light on their role in protein folding and transport and in recognition between Vg and its target organ, the oocyte. |
| Starting Page | 159 |
| Ending Page | 169 |
| Page Count | 11 |
| File Format | PDF HTM / HTML |
| DOI | 10.1007/s10719-009-9268-3 |
| PubMed reference number | 19921429 |
| Journal | Medline |
| Volume Number | 27 |
| Alternate Webpage(s) | http://lifeserv.bgu.ac.il/wb/sagia/media/Articles/77_Roth_et_al_2010_Glycoconj_J_27_159-169_.pdf |
| Journal | Glycoconjugate Journal |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
