Hepatoprotective and antioxidant effects of lycopene on non-alcoholic fatty liver disease in rat

Content Provider	Semantic Scholar
Author	Jiang, Wei Guo, Mei-Hua Hai, Xin
Copyright Year	2016
Abstract	AIM To evaluate the hepatoprotective effect of lycopene (Ly) on non-alcoholic fatty liver disease (NAFLD) in rat. METHODS A rat model of NAFLD was first established by feeding a high-fat diet for 14 wk. Sixty-five rats were randomly divided into normal group, model group and Ly treatment groups. Alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglycerides (TG), total cholesterol (TC) in serum and low density lipoprotein-cholesterol (LDL-C), high density lipoprotein-cholesterol (HDL-C), free fatty acid (FFA), malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH) in liver tissue were evaluated, respectively. While the hepatoprotective effect was also confirmed by histopathological analysis, the expression levels of TNF-α and cytochrome P450 (CYP) 2E1 in rat liver were determined by immunohistochemistry analysis. RESULTS A significant decrease was observed in the levels of serum AST (2.07-fold), ALT (2.95-fold), and the blood lipid TG (2.34-fold) and TC (1.66-fold) in the dose of 20 mg/kg Ly-treated rats (P < 0.01), compared to the model group. Pretreatment with 5, 10 and 20 mg/kg of Ly significantly raised the levels of antioxidant enzyme SOD in a dose-dependent manner, to 90.95 ± 9.56, 109.52 ± 11.34 and 121.25 ± 10.68 (P < 0.05, P < 0.01), as compared with the model group. Similarly, the levels of GSH were significantly increased (P < 0.05, P < 0.01) after the Ly treatment. Meanwhile, pretreatment with 5, 10 and 20 mg/kg of Ly significantly reduced MDA amount by 30.87, 45.51 and 54.49% in the liver homogenates, respectively (P < 0.01). The Ly treatment group showed significantly decreased levels of lipid products LDL-C (P < 0.05, P < 0.01), improved HDL-C level and significantly decreased content of FFA, compared to the model group (P < 0.05, P < 0.01). Furthermore, the Ly-treated group also exhibited a down-regulated TNF-α and CYP2E1 expression, decreased infiltration of liver fats and reversed histopathological changes, all in a dose-dependent manner (P < 0.05, P < 0.01). CONCLUSION This study suggests that Ly has a protective effect on NAFLD, down-regulates expression of TNF-α, and that CYP2E1 may be one of the action mechanisms for Ly.
Starting Page	10180
Ending Page	10188
Page Count	9
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/62/4c/WJG-22-10180.PMC5155177.pdf
PubMed reference number	28028366v1
Alternate Webpage(s)	https://doi.org/10.3748/wjg.v22.i46.10180
DOI	10.3748/wjg.v22.i46.10180
Journal	World journal of gastroenterology
Volume Number	22
Language	English
Access Restriction	Open
Subject Keyword	3,4-Methylenedioxyamphetamine Alanine Angiotensin-Converting Enzyme Inhibitors Antioxidants Aspartate Transaminase Aspartic Acid Cholesterol Cytochrome P-450 CYP2E1 Diet, High-Fat Fatty Liver, Alcoholic Fatty acid glycerol esters Glutathione Hematological Disease High Density Lipoproteins Liver diseases Liver parenchyma Low-Density Lipoproteins Malondialdehyde Non-alcoholic Fatty Liver Disease Nonesterified Fatty Acids Superoxide Dismutase Superoxides Transaminases Triglycerides downregulation of ferrisuperoxide dismutase activity lycopene mg/kg
Content Type	Text
Resource Type	Article