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Novel 5' exon of scavenger receptor CD36 is expressed in cultured human vascular smooth muscle cells and atherosclerotic plaques.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Zingg, J. M. Ricciarelli, Roberta Andorno, Enzo Azzi, Angelo |
| Copyright Year | 2002 |
| Abstract | CD36, a member of the scavenger receptor family, is centrally involved in the uptake of oxidized low density lipoproteins (oxLDLs) from the bloodstream. During the atherosclerotic process, the lipid cargo of oxLDL accumulates in macrophages and smooth muscle cells (SMCs), inducing their pathological conversion to foam cells. Increased expression of CD36 occurs in human atherosclerotic lesions, and CD36 knockout mice show reduced uptake of modified LDLs and reduced atherosclerosis. Here, we describe a novel exon 1b and extended CD36 promoter in human SMCs. Exon 1b is specifically transcribed in activated aortic SMCs and mainly expressed in atherosclerotic plaques. Thus, switching to exon 1b transcription may be an important step for the activation of SMCs and their conversion to foam cells. Using an antisense oligonucleotide to exon 1b, we inhibit CD36 translation and highly reduce oxLDL uptake. The antisense to exon 1b does not affect CD36 in cell lines not expressing the new exon. The possibility of a novel antiatherosclerotic therapy and the use of exon 1b as a marker of atherosclerosis are discussed. |
| Starting Page | 1588 |
| Ending Page | 1594 |
| Page Count | 7 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://atvb.ahajournals.org/content/atvbaha/22/3/412.full.pdf?download=true |
| PubMed reference number | 11884283v1 |
| Volume Number | 22 |
| Issue Number | 3 |
| Journal | Arteriosclerosis, thrombosis, and vascular biology |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Atherosclerosis Blood Circulation Cultured Cell Line Gas Scavengers LErafAON Low-Density Lipoproteins Muscle Cells Muscle, Smooth, Vascular Myocytes, Smooth Muscle Plaque, Atherosclerotic Senile Plaques Smooth muscle (tissue) antisense therapy oxidized low density lipoprotein scavenger receptor |
| Content Type | Text |
| Resource Type | Article |
