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Complete deletion of Wheat streak mosaic virus HC-Pro: a null mutant is viable for systemic infection.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Stenger, Drake C. French, Roy Gildow, Frederick E. |
| Copyright Year | 2005 |
| Abstract | A Wheat streak mosaic virus (WSMV) genome lacking HC-Pro was constructed and confirmed by reverse transcription-PCR to systemically infect wheat, oat, and corn. Coupled in vitro transcription/translation reactions indicated that WSMV P1 proteinase cleaved the polyprotein at the P1/P3 junction of the HC-Pro null mutant. The WSMV HC-Pro null mutant was competent for virion formation, but the virus titer was reduced 4.5-fold relative to that of the wild type. Collectively, these results indicate that WSMV HC-Pro is dispensable for replication and movement, two essential processes that are disrupted by point and small-insertion mutations introduced into potyvirus HC-Pro. |
| File Format | PDF HTM / HTML |
| DOI | 10.1128/JVI.79.18.12077-12080.2005 |
| Alternate Webpage(s) | http://digitalcommons.unl.edu/cgi/viewcontent.cgi?article=1014&context=plantpathpapers |
| PubMed reference number | 16140783 |
| Alternate Webpage(s) | https://doi.org/10.1128/JVI.79.18.12077-12080.2005 |
| Journal | Medline |
| Volume Number | 79 |
| Issue Number | 18 |
| Journal | Journal of virology |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |