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Rapid detection of functional expression of C-5-DNA methyltransferases in yeast.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Kladde, Michael P. Simpson, R. T. |
| Copyright Year | 1998 |
| Abstract | We have previously employed the cytosine-5-DNA methyltransferase (MTase), M. Sss I, as a probe for chromatin architecture in intact cells. Although M. Sss I offers the highest resolution of any currently available MTase, the difficulty in establishing stable, methylation-positive strains poses a barrier to its general utility as a chromatin probe. We describe a simple screen for M. Sss I-expressing strains that eliminates the purification of PCR products amplified from bisulfite-treated DNA, use of radioisotopes, polyacrylamide sequencing gel electrophoresis, and autoradiography. The high throughput of the method now makes it feasible to introduce M. Sss I into a variety of wild-type and mutant genetic backgrounds. |
| Starting Page | 336 |
| Ending Page | 341 |
| Page Count | 6 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://oup.silverchair-cdn.com/oup/backfile/Content_public/Journal/nar/26/5/10.1093/nar/26.5.1354/2/26-5-1354.pdf?Expires=1492201913&Key-Pair-Id=APKAIUCZBIA4LVPAVW3Q&Signature=Mcx1sP~iQshI~BABtzohwMhX9PjhuyESNJpwTWK3Gsb6SMQHBTFI~vx5aEzwBT8QQWc1ry0yOW-F2VyirEc3wxh7F7a3OiHwyr5gC-AMyLs7HBQUT87ohb8Kq8TobmS~XpSH7NWospCVOSQgZHGgyzvkChkasFI0E2TDiQAZ7cLcelqB0Ad2Xz-injKOO5E3ENbnB~A~0E2OPPerEAVdRV7Y8rgGnPJwjHylauzFx8HBFayQAbJReJIgx5asAi48a55bB9kr3uLOzoqfqJfHa6Lm5EduuQ58Om-KorMrGkE46fFt1iEtt~UIviwj79vSnDvNUH3yEARiac~sojMvAw__ |
| PubMed reference number | 9469849v1 |
| Volume Number | 26 |
| Issue Number | 5 |
| Journal | Nucleic acids research |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Autoradiography Biopolymer Sequencing Gel Electrophoresis (lab technique) Genetic Background Methyltransferase Radioisotopes hydrogen sulfite polyacrylamide |
| Content Type | Text |
| Resource Type | Article |
