NDLI: Sequences upstream of the branch site are required to form helix II between U2 and U6 snRNA in a trans-splicing reaction.

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Sequences upstream of the branch site are required to form helix II between U2 and U6 snRNA in a trans-splicing reaction.

Content Provider	Semantic Scholar
Author	Ast, Gil Pavelitz, Thomas Weiner, Alan M.
Copyright Year	2001
Abstract	Three different base paired stems form between U2 and U6 snRNA over the course of the mRNA splicing reaction (helices I, II and III). One possible function of U2/U6 helix II is to facilitate subsequent U2/U6 helix I and III interactions, which participate directly in catalysis. Using an in vitro trans-splicing assay, we investigated the function of sequences located just upstream from the branch site (BS). We find that these upstream sequences are essential for stable binding of U2 to the branch region, and for U2/U6 helix II formation, but not for initial U2/BS pairing. We also show that non-functional upstream sequences cause U2 snRNA stem-loop IIa to be exposed to dimethylsulfate modification, perhaps reflecting a U2 snRNA conformational change and/or loss of SF3b proteins. Our data suggest that initial binding of U2 snRNP to the BS region must be stabilized by an interaction with upstream sequences before U2/U6 helix II can form or U2 stem-loop IIa can participate in spliceosome assembly.
Starting Page	199
Ending Page	200
Page Count	2
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://oup.silverchair-cdn.com/oup/backfile/Content_public/Journal/nar/29/8/10.1093_nar_29.8.1741/1/291741.pdf?Expires=1493011250&Key-Pair-Id=APKAIUCZBIA4LVPAVW3Q&Signature=Uqqfb-Y2-7xH3F3A~5d4ItDOzfW3EDENqv93fvmsY1Zms30yHeKpG8RukMtwV2ZUhS2b9MKUiT-bxlamxqO~C2iVy0kzK2IzkVXJ~5lM5L~buyRBjo0PHRZQQS1RGsVDNRmNsrLTjag9DJS62Ng6p8O6yodgnfJXiYKWooJ9t8UGvLPtU5zIUf1JsprzJ~SP0j6jH7slTtcG6PfUBAdBof-Qw7Et5CEw7iycxROghFn5147MFWdUSB1dvG0KAqSct21PW9GC8gE0SCU2~v9eEb5e1aTJMUETE6poDy0y~LUiZWK-WJhNUXYsxqyxPwrnYTRhpKoRD454qFCXNSjeWQ__
PubMed reference number	11292847v1
Volume Number	29
Issue Number	8
Journal	Nucleic acids research
Language	English
Access Restriction	Open
Subject Keyword	Bundle-Branch Block Catalysis RNA Splicing RNU2-1 gene Ribonucleoprotein, U2 Small Nuclear Small Nuclear RNA Spliceosome Assembly Pathway Spliceosomes Trans-Splicing dimethyl sulfate
Content Type	Text
Resource Type	Article

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