Morphologic and mechanical characteristics of engineered bovine arteries.

Content Provider	Semantic Scholar
Author	Niklason, Laura E. Abbott, W. G. Gao, Jimin Klagges, B. Hirschi, Karen K. Ulubayram, K. Conroy, Nancy E. Jones, Royston D. Vasanawala, A. Sanzgiri, S. M. Langer, Robert
Copyright Year	2001
Abstract	OBJECTIVE The ideal small-caliber arterial graft remains elusive despite several decades of intense research. A novel approach to the development of small-caliber arterial prostheses with a biomimetic system for in vitro vessel culture has recently been described. In this study we examined the effects of culture time and tissue culture scaffolding on engineered vessel morphology and function and found that these parameters greatly influence the function of engineered vessels. METHODS This report describes the effects of culture time and scaffold type on vessel morphology, cellular differentiation, and vessel mechanical characteristics. Engineered vessels were cultured from bovine aortic smooth muscle cells (SMCs) and endothelial cells that were seeded onto biodegradable polymer scaffolds and cultured under physiologically pulsatile conditions. Engineered vessels were subjected to histologic, ultrastructural, immunocytochemical, and mechanical analyses. RESULTS Vessel morphology and mechanical characteristics improved as time in culture increased to 8 weeks. SMCs in the engineered vessel wall were organized into a highly lamellar structure, with cells separated by alternating layers of collagen fibrils. Polymer scaffold remnants were present in vessels cultured for 8 weeks, and SMCs that were in proximity to polymer remnants exhibited a dedifferentiated phenotype. CONCLUSIONS These findings aid in the systematic understanding of the effects of in vitro parameters on engineered vessels and will be useful for the translation of vessel culture techniques to human cells for the development of autologous human vascular grafts.
Starting Page	3018
Ending Page	3023
Page Count	6
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www4.utsouthwestern.edu/gaolab/Pictures/Publication%20pdfs/23_Niklason_J_VAsc_Surg.pdf
PubMed reference number	11241137v1
Volume Number	33
Issue Number	3
Journal	Journal of vascular surgery
Language	English
Access Restriction	Open
Subject Keyword	Arterial system Blood Vessel Tissue Bos taurus Cellular Morphology (discipline) Culture Techniques Diameter (qualifier value) Endothelial Cells Fibril - cell component Histopathologic Grade differentiation In Vitro [Publication Type] Muscle Cells Myocytes, Smooth Muscle Polymers Prosthesis Smooth muscle (tissue) Transplanted tissue Vascular Grafting (surgical procedure) anatomical layer tissue culture
Content Type	Text
Resource Type	Article