NDLI: Somatic Embryogenesis from Root Cambium Tissue of Carrots (Daucus carota L.) by Using Callus and Cell Suspension Culture

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Somatic Embryogenesis from Root Cambium Tissue of Carrots (Daucus carota L.) by Using Callus and Cell Suspension Culture

Content Provider	Semantic Scholar
Author	Günay, Atilla Ellialtıoğlu, Şeküre Şebnem Herdem, Selma Büyükdinç, Damla Turan
Copyright Year	2017
Abstract	Callus and cell suspension cultures provide significant advantages to reveal somaclonal variation in plant breeding studies. When mutagen treatments are integrated, tissue culture techniques can be used to increase the genetic variation to higher frequencies. Callus tissues may provide advantages for mutation applications in breeding studies of carrot to be performed in vitro. For this, optimizing the plant regeneration system from callus and cell suspensions is the first step necessary. Carrot is a plant species which has quite high ability of somatic embryogenesis. In this research aimed at establishing a basis for breeding purposes, tissue fractions containing cambium from the Nantes variety of ripe carrot roots were cultured in MS medium containing different doses of 2,4-D on aseptic conditions. The formation of shoots was observed from callus tissues transplanted to 2,4-D and Kinetin combinations. Callus tissue obtained from medium containing 0.5 mg/L 2,4-D was used to obtain cell suspensions. Two different treatment procedures were applied for the cell suspension. After subcultures containing 1 mg/L 2,4-D, cell clusters are formed in callus suspensions transferred to hormone-free liquid or agar media. Somatic embryogenesis was obtained in agar supplemented media. The method for callus regeneration obtained from the root cambium cells has been successfully applied.
Starting Page	43
Ending Page	48
Page Count	6
File Format	PDF HTM / HTML
Volume Number	1
Alternate Webpage(s)	http://dergipark.gov.tr/download/article-file/423047
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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