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Major histocompatibility complex-mismatched allogeneic bone marrow transplantation using perforin and/or Fas ligand double-defective CD4(+) donor T cells: involvement of cytotoxic function by donor lymphocytes prior to graft-versus-host disease pathogenesis.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Jiang, Zhe Podack, E. Levy, Robert H. |
| Copyright Year | 2001 |
| Abstract | Experimental allogeneic bone marrow transplantation (BMT) models using cytotoxic single-deficient (perforin/granzyme or Fas ligand [FasL]) and cytotoxic double-deficient (cdd) CD4(+) donor T cells have previously demonstrated roles for both effector pathways in graft-versus-host disease (GVHD). In the present study, the role of CD4-mediated antihost cytotoxicity in a GVH response is further examined across a complete major histocompatibility complex class I/II mismatch. As predicted, a double cytotoxic deficiency resulted in a clear delay in GVH-associated weight loss, clinical changes, and mortality. Interestingly, analysis of donor T-cell presence in 5.5-Gy recipients soon after BMT demonstrated that the double cytotoxic deficiency resulted in a marked decrease in donor CD4 numbers. Transplantation of singularly perforin- or FasL-deficient donor CD4(+) T cells demonstrated that the absence of FasL was responsible for the markedly diminished CD4 number in recipient lymph nodes and spleens soon after BMT. However, increasing recipient total body irradiation conditioning (11.0 Gy) abrogated the decrease in FasL-defective B6-cdd and B6-gld CD4 numbers. Thus, the decrease was not a result of inherent CD4 defects, but was probably attributable to host resistance. Consistent with these observations, transplantation into 11.0-Gy recipients resulted in identical GVH lethality by equal numbers of B6 wild-type, B6-cdd, and B6-gld CD4(+) T-cell inoculum. In total, the findings indicate that aggressive host conditioning lessens the requirement for donor CD4(+) cytotoxic function in GVH responses soon after BMT. The present results thus support the notion of a role for cytotoxic effector function in donor CD4(+) T cells prior to GVH-induced tissue injury. |
| Starting Page | 344 |
| Ending Page | 344 |
| Page Count | 1 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.bloodjournal.org/content/bloodjournal/98/2/390.full.pdf?sso-checked=true |
| PubMed reference number | 11435308v1 |
| Volume Number | 98 |
| Issue Number | 2 |
| Journal | Blood |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Cellular Phone Conditioning (Psychology) DNA Breaks, Double-Stranded Defective Viruses FASLG gene Graft versus host disease prophylaxis/therapy Graft-vs-Host Disease Granzymes Leukemia, B-Cell Ligands Major Histocompatibility Complex PRF1 gene Soft Tissue Injuries Spleen Therapeutic radiology procedure Transplanted tissue Whole-Body Irradiation allogeneic bone marrow transplantation lymph nodes perforin |
| Content Type | Text |
| Resource Type | Article |
