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Bone marrow mesenchymal stem cells in children are capable of osteogenic, chondrogenic, adipogenic, myogenic, and neurogenic differentiation *
| Content Provider | Semantic Scholar |
|---|---|
| Author | Ito, Tadaatsu |
| Copyright Year | 2008 |
| Abstract | Bone marrow mesenchymal stem cells (MSCs) were isolated, expanded, and tested their capacity to proliferate and their ability to undergo osteogenesis, chondrogenesis, adipogenesis, myogenesis, and neurogenesis. Marrow samples were obtained from children with various diseases as part of their diagnostic investigation. Cells were layered on 1.073 g/ml Percoll solution, and centrifuged. Cells were cultured in DMEM containing 10% FBS at 37℃ in 5% O 2 and 5% CO2, with medium changes every 3-4 days. MSCs were purified, cultured for many passages, and proliferated extensively. MSCs cultured in osteoinductive medium containing dexamethasone and b-glycerophosphate, showed a change in morphology, and extensive mineralization occurred. MSCs cultured in chondroinductive medium containing TGF-b3, assumed a compact shape. In adipoinductive culture containing insulin and methyl isobutylxanthine, small lipid vacuoles in the MSCs began to become evident after 3-7 days. Cells cultured in myoinductive medium containing HGF or retinoic acid, were stained positively with antidesmine and anti-myoglobin antibodies. MSCs cultured in neuroinductive medium supplemented with butylated hydroxyanisole and DMSO, assumed neuronal morphological characteristics, elaborating long processes. MSCs could be exclusively isolated and expanded from bone marrow of children, and had ability to undergo osteogenesis, chondrogenesis, adipogenesis, myogenesis, and neurogenesis. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://appsv.main.teikyo-u.ac.jp/tosho/tito33.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
