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Determination of glufosinate residue in tea by liquid chromatography-tandem mass spectrometry coupled with precolumn derivatization
| Content Provider | Semantic Scholar |
|---|---|
| Author | Lin, Yonghui Liu, Zhengcai Yang, Fang Qiu, Yuanjin Liu, Suzhen Su, Zhijiao Zhang, Qiong Xue, Zhimin Fang, Yu |
| Copyright Year | 2012 |
| Abstract | A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was established for the determination of glufosinate (GLUF) residue in tea. The GLUF was extracted with water for 30 min under ultrasonication, and cleaned-up using a C18 solid phase extraction cartridge, then derived using fluorenylmethylchloroformate (FMOC-Cl) in borate buffer for 2 h. The separation was performed on a Kinetex C18 column with the mobile phases of acetonitrile and 5 mmol/L ammonium acetate aqueous solution (containing 0.2% (v/v) formic acid) in a gradient elution mode. The identification and quantification of the GLUF were carried out by MS/MS in negative electrospray ionization (ESI(-)) and multiple reaction monitoring (MRM) mode, the quantification analysis was performed by external standard method. The calibration curve showed good linearity in the range of 2.5 - 50.0 microg/L with the correlation coefficient r2 > 0.999. The limit of quantification (LOQ) was 0.10 mg/kg. The average recoveries of GLUF spiked at 0.10, 0.50 and 1.00 mg/kg levels in tea were between 61.6% and 81.4%, and the relative standard deviations (RSDs) were between 3.2% and 8.4%. The method is simple, rapid, sensitive, accurate and suitable for the confirmation and quantification of GLUF in tea. |
| File Format | PDF HTM / HTML |
| DOI | 10.3724/SP.J.1123.2012.07031 |
| PubMed reference number | 23593883 |
| Journal | Medline |
| Volume Number | 30 |
| Issue Number | 12 |
| Alternate Webpage(s) | http://www.chrom-china.com/fileup/PDF/sp1207031.pdf |
| Journal | Se pu = Chinese journal of chromatography |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
