Phenotypic and Functional Analysis of LCMV gp 33-41-Specific CD 8 T Cells Elicited by Multiple Peptide Immunization in Mice Revealed the Up-regulation of PD-1 Expression on Antigen-Specific CD 8 T Cells

Content Provider	Semantic Scholar
Author	Xu, Lihui Jiang, Yiqun Sun, Jianfang He, Xianhui
Copyright Year	2007
Abstract	The phenotype and function of antigen-specific CD8 T cells are closely associated with the efficacy of a therapeutic vaccination. Here we showed that multiple immunizations with LCMV gp33-41 peptide (KAV) in Freund's adjuvant could induce KAV-specific CD8 T cells with low expression of CD127 and CD62L molecules. The inhibitory receptor PD-1 was also expressed on a substantial part of KAV-specific CD8 T cells, and its expression level on KAV-specific CD8 T cells in spleen and lymph nodes was much higher when compared to those in peripheral blood. Furthermore, KAV-specific CD8 T cells could specifically kill KAV-pulsed target cells in vivo but the efficiency was low. These data suggest that prime-boost vaccination schedule with peptide in Freund's adjuvant can elicit antigen-specific CD8 T cells of effector-like phenotype with partial functional exhaustion, which may only provide short-term protection against the pathogen. Cellular & Molecular Immunology. 2007;4(6):431-437.
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.cmi.ustc.edu.cn/4/6/431.pdf
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article