NDLI: Dynamics of the microtubule oscillator: role of nucleotides and tubulin-MAP interactions.

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Dynamics of the microtubule oscillator: role of nucleotides and tubulin-MAP interactions.

Content Provider	Semantic Scholar
Author	Mandelkow, Eckhard Lange, Gudrun Jagla, A. Spann, U.
Copyright Year	1988
Abstract	Microtubules can be induced to perform synchronous and periodic cycles of assembly and disassembly at constant temperature. The process depends on GTP hydrolysis. Time-resolved X-ray scattering using synchrotron radiation shows a cyclic interconversion of tubulin subunits, microtubules and oligomers (= short protofilament fragments). Oscillations are correlated with conditions that stabilize polymers and destabilize oligomers, and others of opposite effect. Microtubule stabilizers include GTP, Mg2+ or microtubule-associated proteins (MAPs), destabilizers include GDP or elevated ionic strength. K+ at intracellular concentrations noticeably increases the stability of tubulin-MAP oligomers, in contrast to Na+. ATP and the non-hydrolyzable analogue AMP-PNP enhance oscillations by mechanisms that are not directly linked to the role of nucleotide hydrolysis in assembly. We propose a mechanism of oscillations that include oligomers as microtubule disassembly products which transiently lock the protein in an unpolymerizable state; this may point to a role of oligomers in controlling microtubule assembly cycles in cells.
Starting Page	5
Ending Page	6
Page Count	2
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.mpasmb-hamburg.mpg.de/mand-pdf/Dynamics%20of%20the%20microtubule_EMMandelkow_Lange_Jagla_Spann_EMandelkoe.pdf
PubMed reference number	3366117v1
Volume Number	7
Issue Number	2
Journal	The EMBO journal
Language	English
Access Restriction	Open
Subject Keyword	AMP-Activated Protein Kinases Adenosine Triphosphate Analog Disassembly (action) Guanosine Diphosphate Guanosine Triphosphate Ionic Strength Microtubule Depolymerization Process Microtubule-Associated Proteins Microtubules Nucleotides Oscillator Device Component Potassium Sodium Synchrotrons
Content Type	Text
Resource Type	Article

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