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Herpes simplex virus type 1 U(L)34 gene product is required for viral envelopment.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Roller, Richard J. Zhou, Yi-Hua Schnetzer, R. Ferguson, John K. Desalvo, D. |
| Copyright Year | 2000 |
| Abstract | The herpes simplex virus type 1 U(L)34 gene encodes a protein that is conserved in all human herpesviruses. The association of the U(L)34 protein with membranes in the infected cell and its expression as a gamma-1 gene suggest a role in maturation or egress of the virus particle from the cell. To determine the function of this gene product, we have constructed a recombinant virus that fails to express the U(L)34 protein. This recombinant virus, in which the U(L)34 protein coding sequence has been replaced by green fluorescent protein, forms minute plaques and replicates in single-step growth experiments to titers 3 to 5 log orders of magnitude lower than wild-type or repair viruses. On Vero cells, the deletion virus synthesizes proteins of all kinetic classes in normal amounts. Electron microscopic and biochemical analyses show that morphogenesis of the deletion virus proceeds normally to the point of formation of DNA-containing nuclear capsids, but electron micrographs show no enveloped virus particles in the cytoplasm or at the surface of infected cells, suggesting that the U(L)34 protein is essential for efficient envelopment of capsids. |
| Starting Page | 5090 |
| Ending Page | 5093 |
| Page Count | 4 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://jvi.asm.org/content/74/1/117.full.pdf |
| PubMed reference number | 10590098v1 |
| Volume Number | 74 |
| Issue Number | 1 |
| Journal | Journal of virology |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Biologic Development Capsid Class Deletion Mutation Enveloped Virus Genome Encoded Entity Green Fluorescent Proteins Herpes Simplex Infections Herpesviridae Human herpesvirus 1 Kinetics Morphogenesis Murine sarcoma viruses Open Reading Frames Recombinants Senile Plaques Staphylococcal Protein A Tissue membrane Virion orders - HL7PublishingDomain |
| Content Type | Text |
| Resource Type | Article |
