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Characterization of acid-sensing ion channels in dorsal horn neurons of rat spinal cord.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Wu, Lianying Duan, Bo Mei, Yi-De Gao, Jun Chen, Jian-Guo Zhuo, Min Xu, Ling Wu, Mian Xu, Tian-Le |
| Copyright Year | 2004 |
| Abstract | Acid-sensing ion channels (ASICs) are ligand-gated cation channels activated by extracellular protons. In periphery, they contribute to sensory transmission, including that of nociception and pain. Here we characterized ASIC-like currents in dorsal horn neurons of the rat spinal cord and their functional modulation in pathological conditions. Reverse transcriptase-nested PCR and Western blotting showed that three ASIC isoforms, ASIC1a, ASIC2a, and ASIC2b, are expressed at a high level in dorsal horn neurons. Electrophysiological and pharmacological properties of the proton-gated currents suggest that homomeric ASIC1a and/or heteromeric ASIC1a + 2b channels are responsible for the proton-induced currents in the majority of dorsal horn neurons. Acidification-induced action potentials in these neurons were compatible in a pH-dependent manner with the pH dependence of ASIC-like current. Furthermore, peripheral complete Freund's adjuvant-induced inflammation resulted in increased expression of both ASIC1a and ASIC2a in dorsal horn. These results support the idea that the ASICs of dorsal horn neurons participate in central sensory transmission/modulation under physiological conditions and may play important roles in inflammation-related persistent pain. |
| Starting Page | 669 |
| Ending Page | 672 |
| Page Count | 4 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://wulab.cbn.rutgers.edu/_documents/Characterizations%20of%20Acid-.pdf |
| Alternate Webpage(s) | http://ir.sibs.ac.cn/bitstream/331001/1940/2/J.%20Biol.%20Chem.-2004-Wu-43716-24.pdf |
| PubMed reference number | 15302881v1 |
| Volume Number | 279 |
| Issue Number | 42 |
| Journal | The Journal of biological chemistry |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | ASIC2 gene ASIC2 wt Allele Action Potentials Calcium Carbonate 800 MG / Famotidine 10 MG / Magnesium Hydroxide 165 MG Chewable Tablet Cations Freund's Adjuvant Iontophoresis Ligands Nociception PPPS protein, Arabidopsis Pain Pharmaceutical Adjuvants Pharmacology Posterior Horn Cells Protein Isoforms Protons Reverse Transcriptase Polymerase Chain Reaction Structure of posterior gray horn of spinal cord Western Blot Western Blotting |
| Content Type | Text |
| Resource Type | Article |
