The structure at 2 A resolution of Phycocyanin from Gracilaria chilensis and the energy transfer network in a PC-PC complex.

Content Provider	Semantic Scholar
Author	Contreras-Martel, Carlos Matamala, Adelio R. Bruna, Carola Poo-Caamaño, Germán Almonacid, Daniel E. Figueroa, Maximiliano F. Martínez-Oyanedel, José Bunster, Marta C.
Copyright Year	2007
Abstract	Phycocyanin is a phycobiliprotein involved in light harvesting and conduction of light to the reaction centers in cyanobacteria and red algae. The structure of C-phycocyanin from Gracilaria chilensis was solved by X-ray crystallography at 2.0 A resolution in space group P2(1). An interaction model between two PC heterohexamers was built, followed by molecular dynamic refinement. The best model showed an inter-hexamer rotation of 23 degrees . The coordinates of a PC heterohexamer (alphabeta)(6) and of the PC-PC complex were used to perform energy transfer calculations between chromophores pairs using the fluorescence resonance energy transfer approach (FRET). Two main intra PC ((I)beta(3)(82)-->(I)alpha(1)(84)-->(I)alpha(5)(84)-->(I)beta(6)(82) and (I)beta(3)(153)-->(I)beta(5)(153)) and two main inter PC ((I)beta(6)(82)-->(II)beta(3)(82) and (I)beta(5)(153)-->(II)beta(3)(153)) pathways were proposed based on the values of the energy transfer constants calculated for all the chromophore pairs in the hexamer and in the complex.
Starting Page	35
Ending Page	41
Page Count	7
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://orbi.ulg.ac.be/bitstream/2268/111234/1/paper4.pdf
PubMed reference number	17118524v1
Volume Number	125
Issue Number	2-3
Journal	Biophysical chemistry
Language	English
Access Restriction	Open
Subject Keyword	Algae Cyanobacteria Data Collection Fatigue Fluorescence Resonance Energy Transfer Gracilaria Molecular Dynamics Phycobilisomes Phycocyanin Rhodophyta Zearaja chilensis monomer
Content Type	Text
Resource Type	Article