NDLI: Accessory-free quantitative smartphone imaging of colorimetric paper-based assays.

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Accessory-free quantitative smartphone imaging of colorimetric paper-based assays.

Content Provider	Semantic Scholar
Author	Kong, Tian Zhang, Biao Nguyen, Brian Tarlan, Farhang Jarvi, Keith A. Sinton, David
Copyright Year	2019
Abstract	The combination of smartphone technology and colorimetric paper-based microfluidics can enable simple, inexpensive diagnostics. However, imaging colorimetric diagnostic results via smartphones currently requires accessories to mitigate the influence of variability in surrounding lighting conditions. Here, we present an accessory-free smartphone-based colorimetric imaging method that enlists the built-in LED light source to dominate ambient lighting in combination with background and colour rescaling. This simple approach enables quantitative measurements from paper-based tests by compensating for different environmental lighting conditions and is universally applicable with respect to phone models and manufacturers. We demonstrate the method with three dominant phone makes and models in a cell counting application with a paper-based yeast detection device. The detection results are in good agreement with cell counting using automated cell counters. Eliminating the need for make/model specific accessories, this approach helps realize the potential for low-cost, broadly applicable paper-based diagnostics.
Starting Page	1991
Ending Page	1999
Page Count	9
File Format	PDF HTM / HTML
DOI	10.1039/c9lc00165d
PubMed reference number	31044203
Journal	Medline
Volume Number	19
Issue Number	11
Alternate Webpage(s)	http://www.rsc.org/suppdata/c9/lc/c9lc00165d/c9lc00165d1.pdf
Alternate Webpage(s)	https://doi.org/10.1039/c9lc00165d
Journal	Lab on a chip
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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